Generation of Induced Pluripotent Stem Cells from Human Nasal Epithelial Cells Using a Sendai Virus Vector

The generation of induced pluripotent stem cells (iPSCs) by introducing reprogramming factors into somatic cells is a promising method for stem cell therapy in regenerative medicine. Therefore, it is desirable to develop a minimally invasive simple method to create iPSCs. In this study, we generated...

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Veröffentlicht in:PloS one Jg. 7; H. 8; S. e42855
Hauptverfasser: Ono, Mizuho, Hamada, Yuko, Horiuchi, Yasue, Matsuo-Takasaki, Mami, Imoto, Yoshimasa, Satomi, Kaishi, Arinami, Tadao, Hasegawa, Mamoru, Fujioka, Tsuyoshi, Nakamura, Yukio, Noguchi, Emiko
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States Public Library of Science 13.08.2012
Public Library of Science (PLoS)
Schlagworte:
Anesthesia
B cells
Biology
Biopsy
Blood
Cellular Reprogramming
DNA methylation
Efficiency
Embryo cells
Embryonic stem cells
Epigenetic inheritance
Epigenetics
Epithelial cells
Female
Fibroblasts
Gene expression
Genes
Genetic vectors
Genetic Vectors - genetics
Genomes
Genomics
Genotypes
Health aspects
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Infections
Lymphocytes
Medical research
Medicine
Molecular modelling
Morphology
Nasal Mucosa - cytology
Otolaryngology
Pathogenesis
Pluripotency
Proteins
Regenerative medicine
Sendai virus - genetics
Somatic cells
Stem cell transplantation
Stem cells
Transduction, Genetic - methods
Vectors
Viruses
Japan
ISSN:1932-6203, 1932-6203
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Zusammenfassung:The generation of induced pluripotent stem cells (iPSCs) by introducing reprogramming factors into somatic cells is a promising method for stem cell therapy in regenerative medicine. Therefore, it is desirable to develop a minimally invasive simple method to create iPSCs. In this study, we generated human nasal epithelial cells (HNECs)-derived iPSCs by gene transduction with Sendai virus (SeV) vectors. HNECs can be obtained from subjects in a noninvasive manner, without anesthesia or biopsy. In addition, SeV carries no risk of altering the host genome, which provides an additional level of safety during generation of human iPSCs. The multiplicity of SeV infection ranged from 3 to 4, and the reprogramming efficiency of HNECs was 0.08-0.10%. iPSCs derived from HNECs had global gene expression profiles and epigenetic states consistent with those of human embryonic stem cells. The ease with which HNECs can be obtained, together with their robust reprogramming characteristics, will provide opportunities to investigate disease pathogenesis and molecular mechanisms in vitro, using cells with particular genotypes.
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Conceived and designed the experiments: Y. Horiuchi MMT TA EN. Performed the experiments: MO Y. Hamada YI KS TF. Analyzed the data: MO TH EN. Contributed reagents/materials/analysis tools: MMT YN MH. Wrote the paper: MO Y. Hamada MMT EN.
Competing Interests: The authors have declared that no competing interests exist.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0042855