Generation of a recombinant Saffold Virus expressing UnaG as a marker for the visualization of viral infection
Background Saffold virus (SAFV), which belongs to the genus Cardiovirus of the family Picornaviridae , is associated with acute respiratory or gastrointestinal illnesses in children; it is also suspected to cause severe diseases, such as acute flaccid paralysis and aseptic meningitis. However, the u...
Saved in:
| Published in: | Virology journal Vol. 20; no. 1; p. 175 |
|---|---|
| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
London
BioMed Central
07.08.2023
BioMed Central Ltd Springer Nature B.V BMC |
| Subjects: | |
| ISSN: | 1743-422X, 1743-422X |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Abstract | Background
Saffold virus (SAFV), which belongs to the genus
Cardiovirus
of the family
Picornaviridae
, is associated with acute respiratory or gastrointestinal illnesses in children; it is also suspected to cause severe diseases, such as acute flaccid paralysis and aseptic meningitis. However, the understanding of the mechanism of its pathogenicity is still limited due to the many unknowns about its lifecycle; for example, the cellular receptor for its infection remains to be determined. A system to monitor SAFV infection in vitro and in vivo is required in order to accelerate research on SAFV.
Results
We generated a recombinant SAFV expressing green fluorescent protein (GFP) or UnaG, a novel fluorescent protein derived from Japanese eel. HeLa cells infected by either GFP or UnaG-expressing SAFV showed a bright green fluorescent signal, enabling convenient monitoring of SAFV infection. However, the expression of GFP but not UnaG was quickly lost during virus passaging due to the difference in genetic stability in the SAFV virus genome; the UnaG gene was stably maintained in the virus genome after at least five passages.
Conclusions
SAFV infection of cultured cells can easily be monitored using UnaG-expressing SAFV, which is superior to GFP in terms of genetic stability in the virus genome. This virus could be a useful tool for SAFV research, such as comparing the susceptibility of various cells to SAFV infection and evaluating the effects of antivirals on SAFV infection in high-throughput screening. |
|---|---|
| AbstractList | BACKGROUND: Saffold virus (SAFV), which belongs to the genus Cardiovirus of the family Picornaviridae, is associated with acute respiratory or gastrointestinal illnesses in children; it is also suspected to cause severe diseases, such as acute flaccid paralysis and aseptic meningitis. However, the understanding of the mechanism of its pathogenicity is still limited due to the many unknowns about its lifecycle; for example, the cellular receptor for its infection remains to be determined. A system to monitor SAFV infection in vitro and in vivo is required in order to accelerate research on SAFV. RESULTS: We generated a recombinant SAFV expressing green fluorescent protein (GFP) or UnaG, a novel fluorescent protein derived from Japanese eel. HeLa cells infected by either GFP or UnaG-expressing SAFV showed a bright green fluorescent signal, enabling convenient monitoring of SAFV infection. However, the expression of GFP but not UnaG was quickly lost during virus passaging due to the difference in genetic stability in the SAFV virus genome; the UnaG gene was stably maintained in the virus genome after at least five passages. CONCLUSIONS: SAFV infection of cultured cells can easily be monitored using UnaG-expressing SAFV, which is superior to GFP in terms of genetic stability in the virus genome. This virus could be a useful tool for SAFV research, such as comparing the susceptibility of various cells to SAFV infection and evaluating the effects of antivirals on SAFV infection in high-throughput screening. Saffold virus (SAFV), which belongs to the genus Cardiovirus of the family Picornaviridae, is associated with acute respiratory or gastrointestinal illnesses in children; it is also suspected to cause severe diseases, such as acute flaccid paralysis and aseptic meningitis. However, the understanding of the mechanism of its pathogenicity is still limited due to the many unknowns about its lifecycle; for example, the cellular receptor for its infection remains to be determined. A system to monitor SAFV infection in vitro and in vivo is required in order to accelerate research on SAFV.BACKGROUNDSaffold virus (SAFV), which belongs to the genus Cardiovirus of the family Picornaviridae, is associated with acute respiratory or gastrointestinal illnesses in children; it is also suspected to cause severe diseases, such as acute flaccid paralysis and aseptic meningitis. However, the understanding of the mechanism of its pathogenicity is still limited due to the many unknowns about its lifecycle; for example, the cellular receptor for its infection remains to be determined. A system to monitor SAFV infection in vitro and in vivo is required in order to accelerate research on SAFV.We generated a recombinant SAFV expressing green fluorescent protein (GFP) or UnaG, a novel fluorescent protein derived from Japanese eel. HeLa cells infected by either GFP or UnaG-expressing SAFV showed a bright green fluorescent signal, enabling convenient monitoring of SAFV infection. However, the expression of GFP but not UnaG was quickly lost during virus passaging due to the difference in genetic stability in the SAFV virus genome; the UnaG gene was stably maintained in the virus genome after at least five passages.RESULTSWe generated a recombinant SAFV expressing green fluorescent protein (GFP) or UnaG, a novel fluorescent protein derived from Japanese eel. HeLa cells infected by either GFP or UnaG-expressing SAFV showed a bright green fluorescent signal, enabling convenient monitoring of SAFV infection. However, the expression of GFP but not UnaG was quickly lost during virus passaging due to the difference in genetic stability in the SAFV virus genome; the UnaG gene was stably maintained in the virus genome after at least five passages.SAFV infection of cultured cells can easily be monitored using UnaG-expressing SAFV, which is superior to GFP in terms of genetic stability in the virus genome. This virus could be a useful tool for SAFV research, such as comparing the susceptibility of various cells to SAFV infection and evaluating the effects of antivirals on SAFV infection in high-throughput screening.CONCLUSIONSSAFV infection of cultured cells can easily be monitored using UnaG-expressing SAFV, which is superior to GFP in terms of genetic stability in the virus genome. This virus could be a useful tool for SAFV research, such as comparing the susceptibility of various cells to SAFV infection and evaluating the effects of antivirals on SAFV infection in high-throughput screening. Saffold virus (SAFV), which belongs to the genus Cardiovirus of the family Picornaviridae, is associated with acute respiratory or gastrointestinal illnesses in children; it is also suspected to cause severe diseases, such as acute flaccid paralysis and aseptic meningitis. However, the understanding of the mechanism of its pathogenicity is still limited due to the many unknowns about its lifecycle; for example, the cellular receptor for its infection remains to be determined. A system to monitor SAFV infection in vitro and in vivo is required in order to accelerate research on SAFV. We generated a recombinant SAFV expressing green fluorescent protein (GFP) or UnaG, a novel fluorescent protein derived from Japanese eel. HeLa cells infected by either GFP or UnaG-expressing SAFV showed a bright green fluorescent signal, enabling convenient monitoring of SAFV infection. However, the expression of GFP but not UnaG was quickly lost during virus passaging due to the difference in genetic stability in the SAFV virus genome; the UnaG gene was stably maintained in the virus genome after at least five passages. SAFV infection of cultured cells can easily be monitored using UnaG-expressing SAFV, which is superior to GFP in terms of genetic stability in the virus genome. This virus could be a useful tool for SAFV research, such as comparing the susceptibility of various cells to SAFV infection and evaluating the effects of antivirals on SAFV infection in high-throughput screening. Saffold virus (SAFV), which belongs to the genus Cardiovirus of the family Picornaviridae, is associated with acute respiratory or gastrointestinal illnesses in children; it is also suspected to cause severe diseases, such as acute flaccid paralysis and aseptic meningitis. However, the understanding of the mechanism of its pathogenicity is still limited due to the many unknowns about its lifecycle; for example, the cellular receptor for its infection remains to be determined. A system to monitor SAFV infection in vitro and in vivo is required in order to accelerate research on SAFV. We generated a recombinant SAFV expressing green fluorescent protein (GFP) or UnaG, a novel fluorescent protein derived from Japanese eel. HeLa cells infected by either GFP or UnaG-expressing SAFV showed a bright green fluorescent signal, enabling convenient monitoring of SAFV infection. However, the expression of GFP but not UnaG was quickly lost during virus passaging due to the difference in genetic stability in the SAFV virus genome; the UnaG gene was stably maintained in the virus genome after at least five passages. SAFV infection of cultured cells can easily be monitored using UnaG-expressing SAFV, which is superior to GFP in terms of genetic stability in the virus genome. This virus could be a useful tool for SAFV research, such as comparing the susceptibility of various cells to SAFV infection and evaluating the effects of antivirals on SAFV infection in high-throughput screening. Background Saffold virus (SAFV), which belongs to the genus Cardiovirus of the family Picornaviridae , is associated with acute respiratory or gastrointestinal illnesses in children; it is also suspected to cause severe diseases, such as acute flaccid paralysis and aseptic meningitis. However, the understanding of the mechanism of its pathogenicity is still limited due to the many unknowns about its lifecycle; for example, the cellular receptor for its infection remains to be determined. A system to monitor SAFV infection in vitro and in vivo is required in order to accelerate research on SAFV. Results We generated a recombinant SAFV expressing green fluorescent protein (GFP) or UnaG, a novel fluorescent protein derived from Japanese eel. HeLa cells infected by either GFP or UnaG-expressing SAFV showed a bright green fluorescent signal, enabling convenient monitoring of SAFV infection. However, the expression of GFP but not UnaG was quickly lost during virus passaging due to the difference in genetic stability in the SAFV virus genome; the UnaG gene was stably maintained in the virus genome after at least five passages. Conclusions SAFV infection of cultured cells can easily be monitored using UnaG-expressing SAFV, which is superior to GFP in terms of genetic stability in the virus genome. This virus could be a useful tool for SAFV research, such as comparing the susceptibility of various cells to SAFV infection and evaluating the effects of antivirals on SAFV infection in high-throughput screening. Background Saffold virus (SAFV), which belongs to the genus Cardiovirus of the family Picornaviridae, is associated with acute respiratory or gastrointestinal illnesses in children; it is also suspected to cause severe diseases, such as acute flaccid paralysis and aseptic meningitis. However, the understanding of the mechanism of its pathogenicity is still limited due to the many unknowns about its lifecycle; for example, the cellular receptor for its infection remains to be determined. A system to monitor SAFV infection in vitro and in vivo is required in order to accelerate research on SAFV. Results We generated a recombinant SAFV expressing green fluorescent protein (GFP) or UnaG, a novel fluorescent protein derived from Japanese eel. HeLa cells infected by either GFP or UnaG-expressing SAFV showed a bright green fluorescent signal, enabling convenient monitoring of SAFV infection. However, the expression of GFP but not UnaG was quickly lost during virus passaging due to the difference in genetic stability in the SAFV virus genome; the UnaG gene was stably maintained in the virus genome after at least five passages. Conclusions SAFV infection of cultured cells can easily be monitored using UnaG-expressing SAFV, which is superior to GFP in terms of genetic stability in the virus genome. This virus could be a useful tool for SAFV research, such as comparing the susceptibility of various cells to SAFV infection and evaluating the effects of antivirals on SAFV infection in high-throughput screening. Keywords: Saffold virus, UnaG BackgroundSaffold virus (SAFV), which belongs to the genus Cardiovirus of the family Picornaviridae, is associated with acute respiratory or gastrointestinal illnesses in children; it is also suspected to cause severe diseases, such as acute flaccid paralysis and aseptic meningitis. However, the understanding of the mechanism of its pathogenicity is still limited due to the many unknowns about its lifecycle; for example, the cellular receptor for its infection remains to be determined. A system to monitor SAFV infection in vitro and in vivo is required in order to accelerate research on SAFV.ResultsWe generated a recombinant SAFV expressing green fluorescent protein (GFP) or UnaG, a novel fluorescent protein derived from Japanese eel. HeLa cells infected by either GFP or UnaG-expressing SAFV showed a bright green fluorescent signal, enabling convenient monitoring of SAFV infection. However, the expression of GFP but not UnaG was quickly lost during virus passaging due to the difference in genetic stability in the SAFV virus genome; the UnaG gene was stably maintained in the virus genome after at least five passages.ConclusionsSAFV infection of cultured cells can easily be monitored using UnaG-expressing SAFV, which is superior to GFP in terms of genetic stability in the virus genome. This virus could be a useful tool for SAFV research, such as comparing the susceptibility of various cells to SAFV infection and evaluating the effects of antivirals on SAFV infection in high-throughput screening. Abstract Background Saffold virus (SAFV), which belongs to the genus Cardiovirus of the family Picornaviridae, is associated with acute respiratory or gastrointestinal illnesses in children; it is also suspected to cause severe diseases, such as acute flaccid paralysis and aseptic meningitis. However, the understanding of the mechanism of its pathogenicity is still limited due to the many unknowns about its lifecycle; for example, the cellular receptor for its infection remains to be determined. A system to monitor SAFV infection in vitro and in vivo is required in order to accelerate research on SAFV. Results We generated a recombinant SAFV expressing green fluorescent protein (GFP) or UnaG, a novel fluorescent protein derived from Japanese eel. HeLa cells infected by either GFP or UnaG-expressing SAFV showed a bright green fluorescent signal, enabling convenient monitoring of SAFV infection. However, the expression of GFP but not UnaG was quickly lost during virus passaging due to the difference in genetic stability in the SAFV virus genome; the UnaG gene was stably maintained in the virus genome after at least five passages. Conclusions SAFV infection of cultured cells can easily be monitored using UnaG-expressing SAFV, which is superior to GFP in terms of genetic stability in the virus genome. This virus could be a useful tool for SAFV research, such as comparing the susceptibility of various cells to SAFV infection and evaluating the effects of antivirals on SAFV infection in high-throughput screening. |
| ArticleNumber | 175 |
| Audience | Academic |
| Author | Higuchi, Masaya Utani, Koichi Himeda, Toshiki Okuwa, Takako |
| Author_xml | – sequence: 1 givenname: Takako surname: Okuwa fullname: Okuwa, Takako organization: Department of Microbiology, Kanazawa Medical University School of Medicine – sequence: 2 givenname: Toshiki surname: Himeda fullname: Himeda, Toshiki organization: Department of Microbiology, Kanazawa Medical University School of Medicine – sequence: 3 givenname: Koichi surname: Utani fullname: Utani, Koichi organization: Department of Microbiology, Kanazawa Medical University School of Medicine – sequence: 4 givenname: Masaya surname: Higuchi fullname: Higuchi, Masaya email: masahigu@kanazawa-med.ac.jp organization: Department of Microbiology, Kanazawa Medical University School of Medicine |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/37550694$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFkl1v0zAUhiM0xD7gD3CBInEDFxn-dnKFpglKpUlIjCHuLMc-6VxSu9hJNfj1uO3o1gkNxVGsk-d9c3zyHhcHPngoipcYnWJci3cJk6bmFSI035iRqn5SHGHJaMUI-X5wb39YHKc0R4gSIZtnxSGVnCPRsKPCT8BD1IMLvgxdqcsIJixa57UfykvddaG35TcXx1TCzTJCSs7PyiuvJ6VOGV_o-ANi2YVYDtdQrlwade9-7wxXLuq-dL4Dsy49L552uk_w4vZ5Ulx9_PD1_FN18XkyPT-7qIxkeKhazqnpLONUC4sa4BKDZBoQMGBtR1BrbVvXLW8aBlS3QhjKCbcSatISA_SkmG59bdBztYwu9_lLBe3UphDiTOk4ONODAqM7C5y1gnYMJK51bbGwWgthpaBrr_dbr-XYLsAa8EM-057p_hvvrtUsrBRGDNUYNdnhza1DDD9HSINauGSg77WHMCZFMc9LMI7-i5KaSckEEiKjrx-g8zBGn8eaKU4oIoLgO2qm82Hzjwi5R7M2VWdSoBwe1pBMnf6DypeFhTM5dp3L9T3B2z1BZga4GWZ6TElNL7_ss6_uD3A3ub8pzADZAiaGlCJ0OwQjtY662kZdZUO1ibqqs6h-IDJu2OQut-76x6V0K035O34G8W5yj6j-AJuaEbY |
| CitedBy_id | crossref_primary_10_3390_molecules30030439 crossref_primary_10_1021_acssensors_5c01213 |
| Cites_doi | 10.3201/eid1801.110725 10.1016/S0042-6822(03)00245-9 10.1002/jbio.202000182 10.3201/eid1405.071675 10.1002/jmv.24928 10.1099/vir.0.066498-0 10.1002/j.1460-2075.1994.tb06337.x 10.1371/journal.ppat.1000416 10.1128/JVI.01616-19 10.1099/vir.0.83428-0 10.7883/yoken.JJID.2015.488 10.1592/phco.23.3.339.32099 10.1128/JVI.00746-16 10.1016/j.cell.2013.05.038 10.1016/j.jcv.2015.06.100 10.1371/journal.pone.0053194 10.3201/eid1409.080570 10.1128/JVI.01043-15 10.1186/1743-422X-8-110 10.1016/j.jcv.2015.11.028 10.1128/JCM.00174-07 10.1073/pnas.1102932108 10.1016/j.jcv.2013.03.015 |
| ContentType | Journal Article |
| Copyright | The Author(s) 2023 2023. BioMed Central Ltd., part of Springer Nature. COPYRIGHT 2023 BioMed Central Ltd. 2023. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. BioMed Central Ltd., part of Springer Nature 2023 |
| Copyright_xml | – notice: The Author(s) 2023 – notice: 2023. BioMed Central Ltd., part of Springer Nature. – notice: COPYRIGHT 2023 BioMed Central Ltd. – notice: 2023. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: BioMed Central Ltd., part of Springer Nature 2023 |
| DBID | C6C AAYXX CITATION NPM ISR 3V. 7U9 7X7 7XB 88E 8FI 8FJ 8FK ABUWG AFKRA AZQEC BENPR CCPQU DWQXO FYUFA GHDGH H94 K9. M0S M1P PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQQKQ PQUKI 7X8 7S9 L.6 5PM DOA |
| DOI | 10.1186/s12985-023-02142-8 |
| DatabaseName | Springer Nature OA/Free Journals CrossRef PubMed Gale In Context: Science ProQuest Central (Corporate) Virology and AIDS Abstracts Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni Edition) ProQuest Central UK/Ireland ProQuest Central Essentials ProQuest Central ProQuest One Community College ProQuest Central Korea Health Research Premium Collection Health Research Premium Collection (Alumni) AIDS and Cancer Research Abstracts ProQuest Health & Medical Complete (Alumni) Health & Medical Collection (Alumni Edition) Medical Database ProQuest Central Premium ProQuest One Academic (New) Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic (retired) ProQuest One Academic UKI Edition MEDLINE - Academic AGRICOLA AGRICOLA - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals |
| DatabaseTitle | CrossRef PubMed Publicly Available Content Database ProQuest One Academic Middle East (New) ProQuest Central Essentials ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest One Health & Nursing ProQuest Central ProQuest Health & Medical Research Collection Health Research Premium Collection Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Health & Medical Research Collection AIDS and Cancer Research Abstracts ProQuest Central (New) ProQuest Medical Library (Alumni) Virology and AIDS Abstracts ProQuest One Academic Eastern Edition ProQuest Hospital Collection Health Research Premium Collection (Alumni) ProQuest Hospital Collection (Alumni) ProQuest Health & Medical Complete ProQuest Medical Library ProQuest One Academic UKI Edition ProQuest One Academic ProQuest One Academic (New) ProQuest Central (Alumni) MEDLINE - Academic AGRICOLA AGRICOLA - Academic |
| DatabaseTitleList | AGRICOLA MEDLINE - Academic PubMed Publicly Available Content Database |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: PIMPY name: Publicly Available Content Database url: http://search.proquest.com/publiccontent sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine Biology |
| EISSN | 1743-422X |
| EndPage | 175 |
| ExternalDocumentID | oai_doaj_org_article_ecafde54b63f4e718a8d16daa66d763e PMC10408109 A760023492 37550694 10_1186_s12985_023_02142_8 |
| Genre | Journal Article |
| GeographicLocations | United States Japan |
| GeographicLocations_xml | – name: United States – name: Japan |
| GrantInformation_xml | – fundername: Grant for Promoted Research from Kanazawa Medical University grantid: S2015-4, S2017-2, S2021-3 – fundername: JSPS KAKENHI from the Japan Society for the Promotion of Science grantid: JP17K08865, JP21K07045 – fundername: Grant for Assistance KAKEN from Kanazawa Medical University grantid: K2019-7, K2020-3, K2020-4 – fundername: ; grantid: JP17K08865, JP21K07045 – fundername: ; grantid: S2015-4, S2017-2, S2021-3 – fundername: ; grantid: K2019-7, K2020-3, K2020-4 |
| GroupedDBID | --- 0R~ 123 29Q 2WC 53G 5VS 7X7 88E 8FI 8FJ AAFWJ AAHBH AAJSJ AASML ABDBF ABUWG ACGFO ACGFS ACIHN ACMJI ACPRK ACUHS ADBBV ADRAZ ADUKV AEAQA AENEX AFKRA AFPKN AHBYD AHMBA AHYZX ALMA_UNASSIGNED_HOLDINGS AMKLP AMTXH AOIJS BAPOH BAWUL BCNDV BENPR BFQNJ BMC BPHCQ BVXVI C6C CCPQU CS3 DIK E3Z EAD EAP EAS EBD EBLON EBS EMB EMK EMOBN ESX F5P FYUFA GROUPED_DOAJ GX1 HMCUK HYE IAO IGS IHR INH INR ISR ITC KQ8 LGEZI LOTEE M1P M48 M~E NADUK NXXTH O5R O5S OK1 OVT P2P PGMZT PHGZM PHGZT PIMPY PJZUB PPXIY PQQKQ PROAC PSQYO PUEGO RBZ RNS ROL RPM RSV SBL SOJ SV3 TR2 TUS UKHRP WOQ WOW XSB AAYXX AFFHD CITATION ALIPV NPM 3V. 7U9 7XB 8FK AZQEC DWQXO H94 K9. PKEHL PQEST PQUKI 7X8 7S9 L.6 5PM |
| ID | FETCH-LOGICAL-c741t-b553cfd453a6d09e571e74ae0e4e4bf20bddb88b5994e3ab66c3525d7e82b2ce3 |
| IEDL.DBID | RSV |
| ISICitedReferencesCount | 2 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=001043738000001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 1743-422X |
| IngestDate | Fri Oct 03 12:50:27 EDT 2025 Tue Nov 04 02:06:17 EST 2025 Sun Nov 09 12:19:54 EST 2025 Thu Sep 04 15:07:59 EDT 2025 Sun Oct 19 01:30:33 EDT 2025 Tue Nov 11 11:14:36 EST 2025 Tue Nov 04 18:36:29 EST 2025 Wed Nov 26 11:12:11 EST 2025 Mon Jul 21 05:55:32 EDT 2025 Tue Nov 18 20:52:07 EST 2025 Sat Nov 29 03:01:59 EST 2025 Sat Sep 06 07:30:05 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 1 |
| Keywords | Saffold virus UnaG |
| Language | English |
| License | 2023. BioMed Central Ltd., part of Springer Nature. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c741t-b553cfd453a6d09e571e74ae0e4e4bf20bddb88b5994e3ab66c3525d7e82b2ce3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 |
| OpenAccessLink | https://link.springer.com/10.1186/s12985-023-02142-8 |
| PMID | 37550694 |
| PQID | 2852302621 |
| PQPubID | 55248 |
| PageCount | 1 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_ecafde54b63f4e718a8d16daa66d763e pubmedcentral_primary_oai_pubmedcentral_nih_gov_10408109 proquest_miscellaneous_3153156450 proquest_miscellaneous_2847746066 proquest_journals_2852302621 gale_infotracmisc_A760023492 gale_infotracacademiconefile_A760023492 gale_incontextgauss_ISR_A760023492 pubmed_primary_37550694 crossref_primary_10_1186_s12985_023_02142_8 crossref_citationtrail_10_1186_s12985_023_02142_8 springer_journals_10_1186_s12985_023_02142_8 |
| PublicationCentury | 2000 |
| PublicationDate | 2023-08-07 |
| PublicationDateYYYYMMDD | 2023-08-07 |
| PublicationDate_xml | – month: 08 year: 2023 text: 2023-08-07 day: 07 |
| PublicationDecade | 2020 |
| PublicationPlace | London |
| PublicationPlace_xml | – name: London – name: England |
| PublicationTitle | Virology journal |
| PublicationTitleAbbrev | Virol J |
| PublicationTitleAlternate | Virol J |
| PublicationYear | 2023 |
| Publisher | BioMed Central BioMed Central Ltd Springer Nature B.V BMC |
| Publisher_xml | – name: BioMed Central – name: BioMed Central Ltd – name: Springer Nature B.V – name: BMC |
| References | ACY Nielsen (2142_CR11) 2012; 18 H Ito (2142_CR10) 2017; 70 MD Ryan (2142_CR12) 1994; 13 2142_CR20 TS Nielsen (2142_CR9) 2016; 74 Y Liu (2142_CR24) 2020; 13 A Naeem (2142_CR7) 2014; 95 JJ Binder (2142_CR18) 2003; 312 ACY Nielsen (2142_CR6) 2013; 57 JF Drexler (2142_CR5) 2008; 14 TL Lin (2142_CR3) 2015; 70 G Loughran (2142_CR17) 2011; 108 2142_CR19 2142_CR8 GA Luke (2142_CR14) 2008; 89 Y Abed (2142_CR2) 2008; 14 A Kumagai (2142_CR15) 2013; 153 MS Jones (2142_CR1) 2007; 45 LK Finch (2142_CR16) 2015; 89 2142_CR23 T Itagaki (2142_CR4) 2018; 90 E Martinez-Salas (2142_CR13) 2010 E Mullapudi (2142_CR22) 2016; 90 NR Florea (2142_CR21) 2003; 23 |
| References_xml | – volume: 18 start-page: 7 year: 2012 ident: 2142_CR11 publication-title: Emerg Infect Dis doi: 10.3201/eid1801.110725 – volume: 312 start-page: 481 year: 2003 ident: 2142_CR18 publication-title: Virology doi: 10.1016/S0042-6822(03)00245-9 – volume: 13 start-page: e202000182 year: 2020 ident: 2142_CR24 publication-title: J Biophotonics doi: 10.1002/jbio.202000182 – volume: 14 start-page: 834 year: 2008 ident: 2142_CR2 publication-title: Emerg Infect Dis doi: 10.3201/eid1405.071675 – volume: 90 start-page: 34 year: 2018 ident: 2142_CR4 publication-title: J Med Virol doi: 10.1002/jmv.24928 – start-page: 141 volume-title: The picornaviruses year: 2010 ident: 2142_CR13 – volume: 95 start-page: 1945 year: 2014 ident: 2142_CR7 publication-title: J Gen Virol doi: 10.1099/vir.0.066498-0 – volume: 13 start-page: 928 year: 1994 ident: 2142_CR12 publication-title: EMBO J doi: 10.1002/j.1460-2075.1994.tb06337.x – ident: 2142_CR19 doi: 10.1371/journal.ppat.1000416 – ident: 2142_CR23 doi: 10.1128/JVI.01616-19 – volume: 89 start-page: 1036 year: 2008 ident: 2142_CR14 publication-title: J Gen Virol doi: 10.1099/vir.0.83428-0 – volume: 70 start-page: 105 year: 2017 ident: 2142_CR10 publication-title: Jpn J Infect Dis doi: 10.7883/yoken.JJID.2015.488 – volume: 23 start-page: 339 year: 2003 ident: 2142_CR21 publication-title: Pharmacotherapy doi: 10.1592/phco.23.3.339.32099 – volume: 90 start-page: 7628 year: 2016 ident: 2142_CR22 publication-title: J Virol doi: 10.1128/JVI.00746-16 – volume: 153 start-page: 1602 year: 2013 ident: 2142_CR15 publication-title: Cell doi: 10.1016/j.cell.2013.05.038 – volume: 70 start-page: 7 year: 2015 ident: 2142_CR3 publication-title: J Clin Virol doi: 10.1016/j.jcv.2015.06.100 – ident: 2142_CR20 doi: 10.1371/journal.pone.0053194 – volume: 14 start-page: 1398 year: 2008 ident: 2142_CR5 publication-title: Emerg Infect Dis doi: 10.3201/eid1409.080570 – volume: 89 start-page: 8580 year: 2015 ident: 2142_CR16 publication-title: J Virol doi: 10.1128/JVI.01043-15 – ident: 2142_CR8 doi: 10.1186/1743-422X-8-110 – volume: 74 start-page: 78 year: 2016 ident: 2142_CR9 publication-title: J Clin Virol doi: 10.1016/j.jcv.2015.11.028 – volume: 45 start-page: 2144 year: 2007 ident: 2142_CR1 publication-title: J Clin Microbiol doi: 10.1128/JCM.00174-07 – volume: 108 start-page: E1111 year: 2011 ident: 2142_CR17 publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.1102932108 – volume: 57 start-page: 239 year: 2013 ident: 2142_CR6 publication-title: J Clin Virol doi: 10.1016/j.jcv.2013.03.015 |
| SSID | ssj0032679 |
| Score | 2.37499 |
| Snippet | Background
Saffold virus (SAFV), which belongs to the genus
Cardiovirus
of the family
Picornaviridae
, is associated with acute respiratory or gastrointestinal... Saffold virus (SAFV), which belongs to the genus Cardiovirus of the family Picornaviridae, is associated with acute respiratory or gastrointestinal illnesses... Background Saffold virus (SAFV), which belongs to the genus Cardiovirus of the family Picornaviridae, is associated with acute respiratory or gastrointestinal... BackgroundSaffold virus (SAFV), which belongs to the genus Cardiovirus of the family Picornaviridae, is associated with acute respiratory or gastrointestinal... BACKGROUND: Saffold virus (SAFV), which belongs to the genus Cardiovirus of the family Picornaviridae, is associated with acute respiratory or gastrointestinal... Abstract Background Saffold virus (SAFV), which belongs to the genus Cardiovirus of the family Picornaviridae, is associated with acute respiratory or... |
| SourceID | doaj pubmedcentral proquest gale pubmed crossref springer |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 175 |
| SubjectTerms | Amino acids Analysis Anguilla japonica Antiviral agents Antiviral drugs Aseptic meningitis Biomedical and Life Sciences Biomedicine Cardiovirus B Care and treatment Cloning Control Diagnosis Diseases Dosage and administration family Fluorescence Gastrointestinal diseases gastrointestinal system Gene expression Genes genetic stability Genomes Genomics genus Green fluorescent protein HeLa cells High-throughput screening Identification and classification Infections Meningitis Methodology Paralysis Pathogenicity Picornaviruses Proteins Saffold virus Scientific equipment and supplies industry UnaG United States viral genome Viral infections Viral proteins Virology Viruses |
| SummonAdditionalLinks | – databaseName: DOAJ Directory of Open Access Journals dbid: DOA link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwEB6hChAXBOUVWpBBSBxg1TwcxzkWRIEDFaIU9WY5frQrbRO02a0Kv56ZOAlNUeHCZQ_r8So774nH3wC8EFyWpkyxTI2tm3FrDNoc1jw0hVtLab3nYdhEsb8vj47KzxdGfVFPWIAHDozbcUZ763Jeicxzh55US5sIq7UQFm3DkffFrGcopoIPxpykKIcrMlLstBjVJN1EpjPLhKMPmIShDq3_T598IShdbpi8dGraBaO9O3C7zyLZbnj6u3DN1ZtwI8yV_LEJNz_1J-b3oA640sR-1nimGVXAp1XX_8IOtPfNwrJv8-W6Ze48NMXWx-yw1u-ZbpH8lNp3lgxTW4apIjubt3QL8-f4g9QjvGBDS1d9Hw733n19-2HWz1iYGcwlVrMqzzPjLc8zLWxcurxIXMG1ix13vPJpXFlbSVnlZcldpishDAGo2sLJtEqNyx7ARt3U7hEwaYoyzjh9StQAgTss1t6V9qgC3pgIkoHlyvQA5DQHY6G6QkQKFcSkUEyqE5OSEbwa93wP8Bt_pX5DkhwpCTq7-wIVSvUKpf6lUBE8Jz1QBI5RU_fNsV63rfp48EXtdqeYhOcYwcueyDf4H4zuLzMgJwhPa0K5PaFE6zXT5UHdVO89WpVKelefijSJ4Nm4TDupI652zZpoOGbuVH5eTZNhOCMwoDyO4GHQ4JE3WYG1qSh5BHKi2xPmTVfq-UmHP44VPCaScRnB68EMfj_71dJ5_D-kswW30s6M5SwutmFjtVy7J3DdnK3m7fJp5wR-AZMsX6U priority: 102 providerName: Directory of Open Access Journals – databaseName: ProQuest Central dbid: BENPR link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3Nb9MwFLegA8SFj_EVGMggJA4QLR-O45zQhjbgQDVtDPVmOf4olbpkNO0E_PW8lzgdGdouXHqon6va79t-_j1CXnMmCl0kkKZGxobMaA06BzkPduFWQhjnWNdsIh-PxWRSHPgDt8aXVfY2sTXUptZ4Rr6dCDy_THgSvz_9EWLXKLxd9S00rpMNRCpjI7Kxuzc-OOxtMcQmLdoeht0hS5JJ_2xG8O0GPJ3A18l4jxkzsAsD19Qi-P9rp_9yVBeLKC_cpLYOav_u_y7tHrnjQ1O608nSfXLNVpvkZtes8tcmufXFX8M_IFUHVo08pbWjimJafVK2RTX0SDlXzw39NlusGmp_dpW21ZQeV-ojVQ2Qn2BN0IJCvEwh_qRnswafdv5e_yAWHs9pXydWPSTH-3tfP3wKfeOGUEOAsgzLLEu1MyxLFTdRYbM8tjlTNrLMstIlUWlMKUSZFQWzqSo514jKanIrkjLRNn1ERlVd2SeECp0XUcrwU4BYcZhhIKEvlQO5cloHJO55JrVHNcfmGnPZZjeCy47PEvgsWz5LEZC36zmnHabHldS7KAprSsTjbr-oF1Pp1VtarZyxGSt56pgFf6-EiblRinMDFtwG5BUKkkTEjQpLeqZq1TTy89Gh3GmvRhEkMiBvPJGrYQ1a-RcSsBMI0jWg3BpQgknQw-Fe0KQ3SY08l7KAvFwP40wss6tsvUIaBukA5rSX06TgIxFhKIsC8rhTgfXepDkkvLxgARED5Rhs3nCkmn1vQc1j8CYijoqAvOv16Py_X86dp1cv9Rm5nbQaLsIo3yKj5WJln5Mb-mw5axYvvLX4A6Qobz8 priority: 102 providerName: ProQuest |
| Title | Generation of a recombinant Saffold Virus expressing UnaG as a marker for the visualization of viral infection |
| URI | https://link.springer.com/article/10.1186/s12985-023-02142-8 https://www.ncbi.nlm.nih.gov/pubmed/37550694 https://www.proquest.com/docview/2852302621 https://www.proquest.com/docview/2847746066 https://www.proquest.com/docview/3153156450 https://pubmed.ncbi.nlm.nih.gov/PMC10408109 https://doaj.org/article/ecafde54b63f4e718a8d16daa66d763e |
| Volume | 20 |
| WOSCitedRecordID | wos001043738000001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVADU databaseName: BioMedCentral customDbUrl: eissn: 1743-422X dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0032679 issn: 1743-422X databaseCode: RBZ dateStart: 20040101 isFulltext: true titleUrlDefault: https://www.biomedcentral.com/search/ providerName: BioMedCentral – providerCode: PRVAON databaseName: DOAJ Directory of Open Access Journals customDbUrl: eissn: 1743-422X dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0032679 issn: 1743-422X databaseCode: DOA dateStart: 20040101 isFulltext: true titleUrlDefault: https://www.doaj.org/ providerName: Directory of Open Access Journals – providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources customDbUrl: eissn: 1743-422X dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0032679 issn: 1743-422X databaseCode: M~E dateStart: 20040101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre – providerCode: PRVPQU databaseName: Health & Medical Collection customDbUrl: eissn: 1743-422X dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0032679 issn: 1743-422X databaseCode: 7X7 dateStart: 20090101 isFulltext: true titleUrlDefault: https://search.proquest.com/healthcomplete providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central customDbUrl: eissn: 1743-422X dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0032679 issn: 1743-422X databaseCode: BENPR dateStart: 20090101 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: Publicly Available Content Database customDbUrl: eissn: 1743-422X dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0032679 issn: 1743-422X databaseCode: PIMPY dateStart: 20090101 isFulltext: true titleUrlDefault: http://search.proquest.com/publiccontent providerName: ProQuest – providerCode: PRVAVX databaseName: SpringerLink customDbUrl: eissn: 1743-422X dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0032679 issn: 1743-422X databaseCode: RSV dateStart: 20041201 isFulltext: true titleUrlDefault: https://link.springer.com/search?facet-content-type=%22Journal%22 providerName: Springer Nature |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3db9MwELfYBogXPsZXYFQGIfEAFWniOM7jhjbYw6qqZVN5shx_lEpdgpp2Av567pykkMGQ4CVS43PUnO8zd_6ZkJeciUxnEaSpobF9ZrQGnYOcB0_hVkIY51h92EQ6HIrpNBs1m8Kqttu9LUl6S-3VWvC3FXgmgbuJse44YKDHW2QH3J1AdRxPzlr7C_FImrXbY_44r-OCPFL_7_b4F4d0uVnyUsXUO6KjO__3CnfJ7SbwpPu1pNwj12yxS27UR1F-2yU3T5oi-31S1FDUuGK0dFRRTJrPc98yQyfKuXJh6Nl8ua6o_Vr30RYzelqo91RVQH6OHT9LCtEwheiSXswr3Lj5ffNAbCte0LYLrHhATo8OP7770G-OZehrCD9W_TxJYu0MS2LFTZjZJB3YlCkbWmZZ7qIwNyYXIk-yjNlY5ZxrxFw1qRVRHmkbPyTbRVnYx4QKnWZhzPAqQGg4zDCQrufKgdQ4rQMyaFdK6gazHI_OWEifuwgua5ZKYKn0LJUiIK83c77UiB1_pT5AAdhQItq2v1EuZ7JRXmm1csYmLOexYxa8uRJmwI1SnBuwzzYgL1B8JOJpFNiwM1PrqpLHk7Hc94VPhIAMyKuGyJXwDlo1-x-AEwjB1aHc61CCwuvucCulsjE4lYwEft6PeDQIyPPNMM7EJrrClmukYRDsY8Z6NU0MHhDxg5IwII9qwd_wJk4hneUZC4joqESHed2RYv7ZQ5ZD0g-xZ5gF5E2rGT__-9Wr8-TfyJ-SW5FXLtEP0z2yvVqu7TNyXV-s5tWyR7bSaeqvokd2Dg6Ho3HPf4KBX6Pjk9GnnrcjPwDuzWhC |
| linkProvider | Springer Nature |
| linkToHtml | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Jb9QwFLZKy3ZhKVuggEEgDhA1i-M4B4TKUjpqOxrRFg0n49jOMFKblEmnUH4Uv5H3skxJUXvrgcsc4udo7LzVfu97hDzjTCQ6CSBM9Yx1mdEaZA5iHuzCrYQwWcbqZhNxvy-Gw2QwR363tTCYVtnqxEpRm0LjGflyIPD8MuCB_2b_u4tdo_B2tW2hUbPFuj36ASFb-br3Hr7v8yBY_bD9bs1tugq4GqzngZtGUagzw6JQceMlNop9GzNlPcssS7PAS41JhUijJGE2VCnnGiFDTWxFkAbahvDeC2SBAbOLebIw6G0OvrS6H3yhCt0P3XyXBcGwLdMRfLkEyyqwGhrvTX0GeqhjCquOAf_ahb8M48mkzRM3t5VBXL3-v23lDXKtcb3pSi0rN8mczRfJpboZ59EiubzZpBncInkNxo08S4uMKorHBntplTREt1SWFbuGfh5PpiW1P-tM4nxEd3L1kaoSyPcw52lCIR6g4F_Tw3GJpau_Zi_ExOpd2ubB5bfJzrms-w6Zz4vc3iNU6DjxQoa_AsSGwwzDEy9VGchNprVD_JZHpG5Q27F5yK6sojfBZc1XEvhKVnwlhUNezubs15glZ1K_RdabUSLeePWgmIxko76k1SozNmIpDzNmwZ9RwvjcKMW5AQtlHfIUGVciokiOKUsjNS1L2dv6JFeqq18EwXTIi4YoK2ANWjUVILATCELWoVzqUILK093hlrFlo3JLeczVDnkyG8aZmEaY22KKNAzCHYzZT6cJwQdABKXIc8jdWuRmexPGENDzhDlEdISxs3ndkXz8rQJt98FaCt9LHPKqldvj_37617l_9lIfkytr25sbcqPXX39ArgaVdhGuFy-R-YPJ1D4kF_XhwbicPGo0FSVfz1ui_wAPSc5X |
| linkToPdf | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Zb9NAEB5BgYoXjnIZCiwIiQeI6mO9Xj-WI1ABUUVp1bfVeo8QKXWqOKmAX8-Mj1AXioR4yUN2Nopn59jxzHwD8ExwmZs8xjA1tG7ArTGocxjz0BRuLaX1njfDJrLRSB4e5runuvjravcuJdn0NBBKU7nYOra-UXEptir0UpI6iykHGXHU6YtwidPQIIrX9w46W4x3kyzvWmX-uK_njmrU_t9t8ynndLZw8kz2tHZKw-v__zg34Fp7IWXbjQTdhAuu3IArzYjK7xuw_qlNvt-CsoGoppNkM880o2D6qKhLadie9n42texgMl9WzH1r6mvLMdsv9TumKyQ_okqgOcNbMsNbJzuZVNTQ-WP1g1RuPGVddVh5G_aHb7-8fj9oxzUMDF5LFoMiTRPjLU8TLWyYuzSLXMa1Cx13vPBxWFhbSFmkec5dogshDGGx2szJuIiNS-7AWjkr3T1g0mR5mHD6lChMAndYDOML7VGavDEBRN2pKdNimdNIjamqYxopVMNShSxVNUuVDODFas9xg-TxV-pXJAwrSkLhrr-YzceqVWrljPbWpbwQiecOvbyWNhJWayEs2m0XwFMSJUU4GyUV8oz1sqrUzt5ntV0nRAkaMoDnLZGf4TMY3fZFICcImqtHudmjRENg-sudxKrWEFUqlvTaPxZxFMCT1TLtpOK60s2WRMMxCKBI9nyaBD0j4QqlYQB3GyVY8SbJMMwVOQ9A9tSjx7z-Sjn5WkOZR-hDZBTmAbzstOTXfz__dO7_G_ljWN99M1Qfd0YfHsDVuNYzOQizTVhbzJfuIVw2J4tJNX9UG4-fO2dt-g |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Generation+of+a+recombinant+Saffold+Virus+expressing+UnaG+as+a+marker+for+the+visualization+of+viral+infection&rft.jtitle=Virology+journal&rft.au=Okuwa%2C+Takako&rft.au=Himeda%2C+Toshiki&rft.au=Utani%2C+Koichi&rft.au=Higuchi%2C+Masaya&rft.date=2023-08-07&rft.pub=BioMed+Central+Ltd&rft.issn=1743-422X&rft.eissn=1743-422X&rft.volume=20&rft.issue=1&rft_id=info:doi/10.1186%2Fs12985-023-02142-8&rft.externalDocID=A760023492 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1743-422X&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1743-422X&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1743-422X&client=summon |