Characterization of the equine skeletal muscle transcriptome identifies novel functional responses to exercise training

Background Digital gene expression profiling was used to characterize the assembly of genes expressed in equine skeletal muscle and to identify the subset of genes that were differentially expressed following a ten-month period of exercise training. The study cohort comprised seven Thoroughbred race...

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Vydáno v:	BMC genomics Ročník 11; číslo 1; s. 398
Hlavní autoři:	McGivney, Beatrice A, McGettigan, Paul A, Browne, John A, Evans, Alexander CO, Fonseca, Rita G, Loftus, Brendan J, Lohan, Amanda, MacHugh, David E, Murphy, Barbara A, Katz, Lisa M, Hill, Emmeline W
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London BioMed Central 23.06.2010 BioMed Central Ltd Springer Nature B.V BMC
Témata:	Aerobic respiration Animal Genetics and Genomics Animals Biomedical and Life Sciences Biomedical research Exercise Female Food science Gene expression Gene Expression Profiling - methods Gene Library Genetic aspects Genomes Genomics Horses - genetics Horses - physiology Human subjects Humans Life Sciences Male Medical research Messenger RNA Mice Microarrays Microbial Genetics and Genomics Muscle, Skeletal - metabolism Muscle, Skeletal - physiology Muscles Muscular system Physical Conditioning, Animal Physical fitness Physical training Physiological aspects Plant Genetics and Genomics Proteomics Real time Reproducibility of Results Research Article Reverse Transcriptase Polymerase Chain Reaction Rodents Studies Time Factors University colleges Veterinary medicine Ireland MSTN Gene Creatine Kinase Muscle Gene Ontology KEGG Pathway Thoroughbred Horse
ISSN:	1471-2164, 1471-2164
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Shrnutí:	Background Digital gene expression profiling was used to characterize the assembly of genes expressed in equine skeletal muscle and to identify the subset of genes that were differentially expressed following a ten-month period of exercise training. The study cohort comprised seven Thoroughbred racehorses from a single training yard. Skeletal muscle biopsies were collected at rest from the gluteus medius at two time points: T 1 - untrained, (9 ± 0.5 months old) and T 2 - trained (20 ± 0.7 months old). Results The most abundant mRNA transcripts in the muscle transcriptome were those involved in muscle contraction, aerobic respiration and mitochondrial function. A previously unreported over-representation of genes related to RNA processing, the stress response and proteolysis was observed. Following training 92 tags were differentially expressed of which 74 were annotated. Sixteen genes showed increased expression, including the mitochondrial genes ACADVL , MRPS21 and SLC25A29 encoded by the nuclear genome. Among the 58 genes with decreased expression, MSTN , a negative regulator of muscle growth, had the greatest decrease. Functional analysis of all expressed genes using FatiScan revealed an asymmetric distribution of 482 Gene Ontology (GO) groups and 18 KEGG pathways. Functional groups displaying highly significant ( P < 0.0001) increased expression included mitochondrion, oxidative phosphorylation and fatty acid metabolism while functional groups with decreased expression were mainly associated with structural genes and included the sarcoplasm, laminin complex and cytoskeleton. Conclusion Exercise training in Thoroughbred racehorses results in coordinate changes in the gene expression of functional groups of genes related to metabolism, oxidative phosphorylation and muscle structure.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	1471-2164 1471-2164
DOI:	10.1186/1471-2164-11-398