Analyzing and identifying novel B cell epitopes within Toxoplasma gondii GRA4

BACKGROUND: The identification of specific epitopes targeted by the host antibody response is important for understanding the natural response to infection and for the development of epitope-based marker vaccines and diagnostic tools for toxoplasmosis. In this study, Toxoplasma gondii GRA4 epitopes...

Full description

Saved in:
Bibliographic Details
Published in:Parasites & vectors Vol. 7; no. 1; p. 474
Main Authors: Wang, Yanhua, Wang, Guangxiang, Ou, Jiangtao, Yin, Hong, Zhang, Delin
Format: Journal Article
Language:English
Published: London Springer-Verlag 10.10.2014
BioMed Central
BioMed Central Ltd
Springer Nature B.V
BMC
Subjects:
Amino Acid Sequence
amino acids
Analysis
Antibodies
Antibody Specificity
Antigenic determinants
Antigens
Antigens, Protozoan
bioinformatics
Biomedical and Life Sciences
Biomedicine
China
Computational Biology
computer software
Entomology
Enzyme-Linked Immunosorbent Assay
Epitope
epitopes
Epitopes, B-Lymphocyte - chemistry
GRA4
Health aspects
Hogs
hydrophilicity
Immune system
Infectious Diseases
nucleotide sequences
Parasitology
Peptides
Pig antibodies
prediction
Protozoan Proteins - chemistry
Protozoan Proteins - metabolism
Software
swine
tachyzoites
Toxoplasma - metabolism
Toxoplasma gondii
toxoplasmosis
Tropical Medicine
Vaccines
Veterinary Medicine/Veterinary Science
Viral antibodies
Virology
China
Pig antibodies
Toxoplasma gondii
Epitope
GRA4
ISSN:1756-3305, 1756-3305
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:BACKGROUND: The identification of specific epitopes targeted by the host antibody response is important for understanding the natural response to infection and for the development of epitope-based marker vaccines and diagnostic tools for toxoplasmosis. In this study, Toxoplasma gondii GRA4 epitopes were identified using software-based prediction and a synthetic peptide technique. METHODS: The complete GRA4 gene sequence was obtained from T. gondii of the Gansu Jingtai strain of tachyzoites. The potential B cell epitopes of GRA4 was predicted using the PROTEAN subroutine in the DNASTAR software package. The peptides with good hydrophilicity, high accessibility, high flexibility and strong antigenicity were chemically synthesized and assessed by ELISA using pig sera from different time points after infection. RESULTS: The potential B cell epitopes of GRA4 predicted by bioinformatics tools focused on six regions of GRA4, 52–77 aa, 93–112 aa, 127–157 aa, 178–201 aa, 223–252 aa and 314–333 aa. Eleven shorter peptides from the six regions were synthesized and assessed by ELISA using pig sera from different time points after infection. Three of the eleven peptides (amino acids 62–77, 233–252 and 314–333) tested were recognized by all sera. CONCLUSIONS: We precisely located the T. gondii GRA4 epitopes using pig sera collected at different time points after infection. The identified epitopes may be useful for additional studies of epitope-based vaccines and diagnostic reagents.
Bibliography:http://dx.doi.org/10.1186/s13071-014-0474-x
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:1756-3305
1756-3305
DOI:10.1186/s13071-014-0474-x