Identification and Characterization of Ixodes scapularis Antigens That Elicit Tick Immunity Using Yeast Surface Display

Repeated exposure of rabbits and other animals to ticks results in acquired resistance or immunity to subsequent tick bites and is partially elicited by antibodies directed against tick antigens. In this study we demonstrate the utility of a yeast surface display approach to identify tick salivary a...

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Published in:PloS one Vol. 6; no. 1; p. e15926
Main Authors: Schuijt, Tim J., Narasimhan, Sukanya, Daffre, Sirlei, DePonte, Kathleen, Hovius, Joppe W. R., Veer, Cornelis van't, van der Poll, Tom, Bakhtiari, Kamran, Meijers, Joost C. M., Boder, Eric T., van Dam, Alje P., Fikrig, Erol
Format: Journal Article
Language:English
Published: United States Public Library of Science 05.01.2011
Public Library of Science (PLoS)
Subjects:
Acari
Animal bites
Animals
Antibodies
Antigens
Antigens - blood
Antigens - immunology
Antigens - isolation & purification
Antisera
Arachnids
Baking yeast
Biology
Bites
Bites (Injuries)
Borrelia
Breast cancer
Cell cycle
Coagulants
Drosophila
Feeding
Identification
Immune serum
Immunity
Immunization
Immunization - methods
Immunoglobulins
Insect bites
Insects
Internal medicine
Ixodes - immunology
Ixodes ricinus
Ixodidae
Libraries
Medicine
Neurogenesis
Nymph
Pathogens
Peptide Library
Proteins
Rabbits
Saccharomyces cerevisiae
Salivary gland
Salivary Glands - immunology
Sexes
Ticks
Ticks - immunology
Vectors (Biology)
Yeast
Yeasts
Zoonoses
United States > US
Connecticut
ISSN:1932-6203, 1932-6203
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Abstract Repeated exposure of rabbits and other animals to ticks results in acquired resistance or immunity to subsequent tick bites and is partially elicited by antibodies directed against tick antigens. In this study we demonstrate the utility of a yeast surface display approach to identify tick salivary antigens that react with tick-immune serum. We constructed an Ixodes scapularis nymphal salivary gland yeast surface display library and screened the library with nymph-immune rabbit sera and identified five salivary antigens. Four of these proteins, designated P8, P19, P23 and P32, had a predicted signal sequence. We generated recombinant (r) P8, P19 and P23 in a Drosophila expression system for functional and immunization studies. rP8 showed anti-complement activity and rP23 demonstrated anti-coagulant activity. Ixodes scapularis feeding was significantly impaired when nymphs were fed on rabbits immunized with a cocktail of rP8, rP19 and rP23, a hall mark of tick-immunity. These studies also suggest that these antigens may serve as potential vaccine candidates to thwart tick feeding.
AbstractList Repeated exposure of rabbits and other animals to ticks results in acquired resistance or immunity to subsequent tick bites and is partially elicited by antibodies directed against tick antigens. In this study we demonstrate the utility of a yeast surface display approach to identify tick salivary antigens that react with tick-immune serum. We constructed an Ixodes scapularis nymphal salivary gland yeast surface display library and screened the library with nymph-immune rabbit sera and identified five salivary antigens. Four of these proteins, designated P8, P19, P23 and P32, had a predicted signal sequence. We generated recombinant (r) P8, P19 and P23 in a Drosophila expression system for functional and immunization studies. rP8 showed anti-complement activity and rP23 demonstrated anti-coagulant activity. Ixodes scapularis feeding was significantly impaired when nymphs were fed on rabbits immunized with a cocktail of rP8, rP19 and rP23, a hall mark of tick-immunity. These studies also suggest that these antigens may serve as potential vaccine candidates to thwart tick feeding.
Repeated exposure of rabbits and other animals to ticks results in acquired resistance or immunity to subsequent tick bites and is partially elicited by antibodies directed against tick antigens. In this study we demonstrate the utility of a yeast surface display approach to identify tick salivary antigens that react with tick-immune serum. We constructed an Ixodes scapularis nymphal salivary gland yeast surface display library and screened the library with nymph-immune rabbit sera and identified five salivary antigens. Four of these proteins, designated P8, P19, P23 and P32, had a predicted signal sequence. We generated recombinant (r) P8, P19 and P23 in a Drosophila expression system for functional and immunization studies. rP8 showed anti-complement activity and rP23 demonstrated anti-coagulant activity. Ixodes scapularis feeding was significantly impaired when nymphs were fed on rabbits immunized with a cocktail of rP8, rP19 and rP23, a hall mark of tick-immunity. These studies also suggest that these antigens may serve as potential vaccine candidates to thwart tick feeding.Repeated exposure of rabbits and other animals to ticks results in acquired resistance or immunity to subsequent tick bites and is partially elicited by antibodies directed against tick antigens. In this study we demonstrate the utility of a yeast surface display approach to identify tick salivary antigens that react with tick-immune serum. We constructed an Ixodes scapularis nymphal salivary gland yeast surface display library and screened the library with nymph-immune rabbit sera and identified five salivary antigens. Four of these proteins, designated P8, P19, P23 and P32, had a predicted signal sequence. We generated recombinant (r) P8, P19 and P23 in a Drosophila expression system for functional and immunization studies. rP8 showed anti-complement activity and rP23 demonstrated anti-coagulant activity. Ixodes scapularis feeding was significantly impaired when nymphs were fed on rabbits immunized with a cocktail of rP8, rP19 and rP23, a hall mark of tick-immunity. These studies also suggest that these antigens may serve as potential vaccine candidates to thwart tick feeding.
Audience Academic
Author Schuijt, Tim J.
DePonte, Kathleen
Veer, Cornelis van't
Fikrig, Erol
van Dam, Alje P.
van der Poll, Tom
Meijers, Joost C. M.
Bakhtiari, Kamran
Daffre, Sirlei
Narasimhan, Sukanya
Hovius, Joppe W. R.
Boder, Eric T.
AuthorAffiliation 7 Department of Medical Microbiology, Onze Lieve Vrouwe Gasthuis, Amsterdam, The Netherlands
3 Department of Experimental Vascular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
5 Departamento de Parasitologia, Universidade de São Paulo, São Paulo, Brazil
4 Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands
2 Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
New York University School of Medicine, United States of America
1 Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut, United States of America
6 Department of Chemical and Biomolecular of Engineering, University of Tennessee, Knoxville, Tennessee, United States of America
AuthorAffiliation_xml – name: 5 Departamento de Parasitologia, Universidade de São Paulo, São Paulo, Brazil
– name: 3 Department of Experimental Vascular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
– name: 6 Department of Chemical and Biomolecular of Engineering, University of Tennessee, Knoxville, Tennessee, United States of America
– name: 1 Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut, United States of America
– name: 2 Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
– name: 7 Department of Medical Microbiology, Onze Lieve Vrouwe Gasthuis, Amsterdam, The Netherlands
– name: New York University School of Medicine, United States of America
– name: 4 Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands
Author_xml – sequence: 1
  givenname: Tim J.
  surname: Schuijt
  fullname: Schuijt, Tim J.
– sequence: 2
  givenname: Sukanya
  surname: Narasimhan
  fullname: Narasimhan, Sukanya
– sequence: 3
  givenname: Sirlei
  surname: Daffre
  fullname: Daffre, Sirlei
– sequence: 4
  givenname: Kathleen
  surname: DePonte
  fullname: DePonte, Kathleen
– sequence: 5
  givenname: Joppe W. R.
  surname: Hovius
  fullname: Hovius, Joppe W. R.
– sequence: 6
  givenname: Cornelis van't
  surname: Veer
  fullname: Veer, Cornelis van't
– sequence: 7
  givenname: Tom
  surname: van der Poll
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  surname: Meijers
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– sequence: 10
  givenname: Eric T.
  surname: Boder
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– sequence: 11
  givenname: Alje P.
  surname: van Dam
  fullname: van Dam, Alje P.
– sequence: 12
  givenname: Erol
  surname: Fikrig
  fullname: Fikrig, Erol
BackLink https://www.ncbi.nlm.nih.gov/pubmed/21246036$$D View this record in MEDLINE/PubMed
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ContentType Journal Article
Copyright COPYRIGHT 2011 Public Library of Science
2011 Schuijt et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Schuijt et al. 2011
Copyright_xml – notice: COPYRIGHT 2011 Public Library of Science
– notice: 2011 Schuijt et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
– notice: Schuijt et al. 2011
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Conceived and designed the experiments: TJS SN SD CvV JCMM EF. Performed the experiments: TJS SN SD KD KB. Analyzed the data: TJS SN SD JWRH CvV KB JCMM APvD EF. Contributed reagents/materials/analysis tools: ETB JCMM TvdP. Wrote the paper: TJS SN EF.
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StartPage e15926
SubjectTerms Acari
Animal bites
Animals
Antibodies
Antigens
Antigens - blood
Antigens - immunology
Antigens - isolation & purification
Antisera
Arachnids
Baking yeast
Biology
Bites
Bites (Injuries)
Borrelia
Breast cancer
Cell cycle
Coagulants
Drosophila
Feeding
Identification
Immune serum
Immunity
Immunization
Immunization - methods
Immunoglobulins
Insect bites
Insects
Internal medicine
Ixodes - immunology
Ixodes ricinus
Ixodidae
Libraries
Medicine
Neurogenesis
Nymph
Pathogens
Peptide Library
Proteins
Rabbits
Saccharomyces cerevisiae
Salivary gland
Salivary Glands - immunology
Sexes
Ticks
Ticks - immunology
Vectors (Biology)
Yeast
Yeasts
Zoonoses
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Title Identification and Characterization of Ixodes scapularis Antigens That Elicit Tick Immunity Using Yeast Surface Display
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