Identification and Characterization of Ixodes scapularis Antigens That Elicit Tick Immunity Using Yeast Surface Display

Repeated exposure of rabbits and other animals to ticks results in acquired resistance or immunity to subsequent tick bites and is partially elicited by antibodies directed against tick antigens. In this study we demonstrate the utility of a yeast surface display approach to identify tick salivary a...

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Veröffentlicht in:PloS one Jg. 6; H. 1; S. e15926
Hauptverfasser: Schuijt, Tim J., Narasimhan, Sukanya, Daffre, Sirlei, DePonte, Kathleen, Hovius, Joppe W. R., Veer, Cornelis van't, van der Poll, Tom, Bakhtiari, Kamran, Meijers, Joost C. M., Boder, Eric T., van Dam, Alje P., Fikrig, Erol
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States Public Library of Science 05.01.2011
Public Library of Science (PLoS)
Schlagworte:
Acari
Animal bites
Animals
Antibodies
Antigens
Antigens - blood
Antigens - immunology
Antigens - isolation & purification
Antisera
Arachnids
Baking yeast
Biology
Bites
Bites (Injuries)
Borrelia
Breast cancer
Cell cycle
Coagulants
Drosophila
Feeding
Identification
Immune serum
Immunity
Immunization
Immunization - methods
Immunoglobulins
Insect bites
Insects
Internal medicine
Ixodes - immunology
Ixodes ricinus
Ixodidae
Libraries
Medicine
Neurogenesis
Nymph
Pathogens
Peptide Library
Proteins
Rabbits
Saccharomyces cerevisiae
Salivary gland
Salivary Glands - immunology
Sexes
Ticks
Ticks - immunology
Vectors (Biology)
Yeast
Yeasts
Zoonoses
United States > US
Connecticut
ISSN:1932-6203, 1932-6203
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Beschreibung
Zusammenfassung:Repeated exposure of rabbits and other animals to ticks results in acquired resistance or immunity to subsequent tick bites and is partially elicited by antibodies directed against tick antigens. In this study we demonstrate the utility of a yeast surface display approach to identify tick salivary antigens that react with tick-immune serum. We constructed an Ixodes scapularis nymphal salivary gland yeast surface display library and screened the library with nymph-immune rabbit sera and identified five salivary antigens. Four of these proteins, designated P8, P19, P23 and P32, had a predicted signal sequence. We generated recombinant (r) P8, P19 and P23 in a Drosophila expression system for functional and immunization studies. rP8 showed anti-complement activity and rP23 demonstrated anti-coagulant activity. Ixodes scapularis feeding was significantly impaired when nymphs were fed on rabbits immunized with a cocktail of rP8, rP19 and rP23, a hall mark of tick-immunity. These studies also suggest that these antigens may serve as potential vaccine candidates to thwart tick feeding.
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Conceived and designed the experiments: TJS SN SD CvV JCMM EF. Performed the experiments: TJS SN SD KD KB. Analyzed the data: TJS SN SD JWRH CvV KB JCMM APvD EF. Contributed reagents/materials/analysis tools: ETB JCMM TvdP. Wrote the paper: TJS SN EF.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0015926