Finding the right coverage: the impact of coverage and sequence quality on single nucleotide polymorphism genotyping error rates

Restriction‐enzyme‐based sequencing methods enable the genotyping of thousands of single nucleotide polymorphism (SNP) loci in nonmodel organisms. However, in contrast to traditional genetic markers, genotyping error rates in SNPs derived from restriction‐enzyme‐based methods remain largely unknown....

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Bibliographic Details
Published in:	Molecular ecology resources Vol. 16; no. 4; pp. 966 - 978
Main Authors:	Fountain, Emily D., Pauli, Jonathan N., Reid, Brendan N., Palsbøll, Per J., Peery, M. Zachariah
Format:	Journal Article
Language:	English
Published:	England Blackwell Publishing Ltd 01.07.2016 Wiley Subscription Services, Inc
Subjects:	Animals Choloepus Choloepus hoffmanni data collection Datasets ddRAD Diagnostic Errors DNA libraries DNA Restriction Enzymes - metabolism Economic models Enzymes Genetic markers genotyping error genotyping errors Genotyping Techniques - methods High-Throughput Nucleotide Sequencing loci Mendelian incompatibility next-generation sequencing Offspring parentage Polymorphism, Single Nucleotide Quality Sequence Analysis, DNA single nucleotide polymorphism Xenarthra - classification Xenarthra - genetics ddRAD genotyping error next-generation sequencing single nucleotide polymorphism Mendelian incompatibility
ISSN:	1755-098X, 1755-0998
Online Access:	Get full text
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