3D printed polylactic acid/gelatin-nano-hydroxyapatite/platelet-rich plasma scaffold for critical-sized skull defect regeneration

Background Three-dimensional (3D) printing is a capable approach for the fabrication of bone tissue scaffolds. Nevertheless, a purely made scaffold such as polylactic acid (PLA) may suffer from shortcomings and be restricted due to its biological behavior. Gelatin, hydroxyapatite and platelet-rich p...

Full description

Saved in:
Bibliographic Details
Published in:Biomedical engineering online Vol. 21; no. 1; pp. 86 - 25
Main Authors: Bahraminasab, Marjan, Doostmohammadi, Nesa, Talebi, Athar, Arab, Samaneh, Alizadeh, Akram, Ghanbari, Ali, Salati, Amir
Format: Journal Article
Language:English
Published: London BioMed Central 12.12.2022
BioMed Central Ltd
Springer Nature B.V
BMC
Subjects:
3D printing
Adhesion
Analysis
Animals
Biocompatibility
Biodegradation
Biomaterials
Biomedical Engineering and Bioengineering
Biomedical Engineering/Biotechnology
Biomedical materials
Biotechnology
Bone growth
Bone healing
Bone regeneration
Calvaria
Cell adhesion
Cell adhesion & migration
Cell proliferation
Compression
Compressive strength
Defects
Degradability
Degradation
Durapatite - chemistry
Engineering
Extracellular matrix
Fabrication
Gelatin
Gelatin - metabolism
Gelatin - pharmacology
Growth factors
Hydroxyapatite
Mechanical properties
Methods
Mineralization
Nano-hydroxyapatite
Osteocalcin
Osteogenesis
Platelet-rich plasma (PRP)
Platelet-Rich Plasma - metabolism
Platelets
Polylactic acid
Porosity
Printing
Printing, Three-Dimensional
Properties
Rats
Regeneration
Regeneration (physiology)
Scaffolds
Scanning electron microscopy
Skull
Three dimensional printing
Tissue engineering
Tissue Engineering - methods
Tissue Scaffolds - chemistry
Transplants & implants
Iran
Platelet-rich plasma (PRP)
Bone regeneration
Gelatin
Nano-hydroxyapatite
3D printing
ISSN:1475-925X, 1475-925X
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background Three-dimensional (3D) printing is a capable approach for the fabrication of bone tissue scaffolds. Nevertheless, a purely made scaffold such as polylactic acid (PLA) may suffer from shortcomings and be restricted due to its biological behavior. Gelatin, hydroxyapatite and platelet-rich plasma (PRP) have been revealed to be of potential to enhance the osteogenic effect. In this study, it was tried to improve the properties of 3D-printed PLA scaffolds by infilling them with gelatin-nano-hydroxyapatite (PLA/G-nHA) and subsequent coating with PRP. For comparison, bare PLA and PLA/G-nHA scaffolds were also fabricated. The printing accuracy, the scaffold structural characterizations, mechanical properties, degradability behavior, cell adhesion, mineralization, systemic effect of the scaffolds on the liver enzymes, osteocalcin level in blood serum and in vivo bone regeneration capability in rat critical-sized calvaria defect were evaluated. Results High printing accuracy (printing error of < 11%) was obtained for all measured parameters including strut thickness, pore width, scaffold density and porosity%. The highest mean ultimate compression strength (UCS) was associated with PLA/G-nHA/PRP scaffolds, which was 10.95 MPa. A slow degradation rate was observed for all scaffolds. The PLA/G-nHA/PRP had slightly higher degradation rate, possibly due to PRP release, with burst release occurred at week 4. The MTT results showed that PLA/G-nHA/PRP provided the highest cell proliferation at all time points, and the serum biochemistry (ALT and AST level) results indicated no abnormal/toxic influence caused by scaffold biomaterials. Superior cell adhesion and mineralization were obtained for PLA/G-nHA/PRP. Furthermore, all the developed scaffolds showed bone repair capability. The PLA/G-nHA/PRP scaffolds could better support bone regeneration than bare PLA and PLA/G-nHA scaffolds. Conclusion The PLA/G-nHA/PRP scaffolds can be considered as potential for hard tissue repair.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:1475-925X
1475-925X
DOI:10.1186/s12938-022-01056-w