Unraveling the structure of DNA during overstretching by using multicolor, single-molecule fluorescence imaging

Single-molecule manipulation studies have revealed that double-stranded DNA undergoes a structural transition when subjected to tension. At forces that depend on the attachment geometry of the DNA (65 pN or 110 pN), it elongates approximately 1.7-fold and its elastic properties change dramatically....

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Bibliographic Details

Published in:	Proceedings of the National Academy of Sciences - PNAS Vol. 106; no. 43; p. 18231
Main Authors:	van Mameren, Joost, Gross, Peter, Farge, Geraldine, Hooijman, Pleuni, Modesti, Mauro, Falkenberg, Maria, Wuite, Gijs J L, Peterman, Erwin J G
Format:	Journal Article
Language:	English
Published:	United States 27.10.2009
Subjects:	DNA - chemistry Microfluidic Analytical Techniques Microscopy, Fluorescence Nucleic Acid Conformation Nucleic Acid Denaturation Optical Tweezers Transition Temperature
ISSN:	1091-6490, 1091-6490
Online Access:	Get more information
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