Temporal dynamics of liver mitochondrial protein acetylation and succinylation and metabolites due to high fat diet and/or excess glucose or fructose

Dietary macronutrient composition alters metabolism through several mechanisms, including post-translational modification (PTM) of proteins. To connect diet and molecular changes, here we performed short- and long-term feeding of mice with standard chow diet (SCD) and high-fat diet (HFD), with or wi...

Celý popis

Uložené v:

Podrobná bibliografia
Vydané v:	PloS one Ročník 13; číslo 12; s. e0208973
Hlavní autori:	Meyer, Jesse G., Softic, Samir, Basisty, Nathan, Rardin, Matthew J., Verdin, Eric, Gibson, Bradford W., Ilkayeva, Olga, Newgard, Christopher B., Kahn, C. Ronald, Schilling, Birgit
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States Public Library of Science 26.12.2018 Public Library of Science (PLoS)
Predmet:	Acetylation Acetylation - drug effects Acylation Aging Amino acids Animals Biology and Life Sciences Citric acid Citric Acid - metabolism Dehydrogenases Diabetes Diet Diet, High-Fat - adverse effects Dietary supplements Enzymes Fat metabolism Fatty acids Fatty Liver - genetics Fatty Liver - metabolism Fatty Liver - pathology Feeding Fructose Glucose Glucose - metabolism Health aspects High fat diet Humans Lipid Metabolism - drug effects Liver Liver - drug effects Liver - metabolism Medicine Medicine and Health Sciences Metabolic syndrome Metabolism Metabolites Metabolomics Mice Mitochondria Mitochondria - drug effects Mitochondria - genetics Mitochondria, Liver - drug effects Mitochondrial Proteins - drug effects Mitochondrial Proteins - genetics Nutrition research Physical Sciences Physiological aspects Physiological research Physiology Post-translation Post-translational modifications Protein Processing, Post-Translational - drug effects Protein Processing, Post-Translational - genetics Protein turnover Proteins Research and Analysis Methods Sugar Translation North Carolina South San Francisco California California United States > US Massachusetts
ISSN:	1932-6203, 1932-6203
On-line prístup:	Získať plný text
Tagy:	Pridať tag Žiadne tagy, Buďte prvý, kto otaguje tento záznam!

Popis
Shrnutí:	Dietary macronutrient composition alters metabolism through several mechanisms, including post-translational modification (PTM) of proteins. To connect diet and molecular changes, here we performed short- and long-term feeding of mice with standard chow diet (SCD) and high-fat diet (HFD), with or without glucose or fructose supplementation, and quantified liver metabolites, 861 proteins, and 1,815 protein level-corrected mitochondrial acetylation and succinylation sites. Nearly half the acylation sites were altered by at least one diet; nutrient-specific changes in protein acylation sometimes encompass entire pathways. Although acetyl-CoA is an intermediate in both sugar and fat metabolism, acetyl-CoA had a dichotomous fate depending on its source; chronic feeding of dietary sugars induced protein hyperacetylation, whereas the same duration of HFD did not. Instead, HFD resulted in citrate accumulation, anaplerotic metabolism of amino acids, and protein hypo-succinylation. Together, our results demonstrate novel connections between dietary macronutrients, protein post-translational modifications, and regulation of fuel selection in liver.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Competing Interests: The authors have declared that no competing interests exist. Current address: Department of Chemistry, Department of Biomolecular Chemistry, National Center for Quantitative Biology of Complex Systems, University of Wisconsin–Madison, Madison, Wisconsin, United States of America
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0208973