Intestinal Microbiota is Different in Women with Preterm Birth: Results from Terminal Restriction Fragment Length Polymorphism Analysis

Preterm birth is a leading cause of perinatal morbidity and mortality. Studies using a cultivation method or molecular identification have shown that bacterial vaginosis is one of the risk factors for preterm birth. However, an association between preterm birth and intestinal microbiota has not been...

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Vydáno v:PloS one Ročník 9; číslo 11; s. e111374
Hlavní autoři: Shiozaki, Arihiro, Yoneda, Satoshi, Yoneda, Noriko, Yonezawa, Rika, Matsubayashi, Takamichi, Seo, Genichiro, Saito, Shigeru
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States Public Library of Science 05.11.2014
Public Library of Science (PLoS)
Témata:
Adult
Analysis
Antibiotics
Asthma
Babies
Bacteria
Bacteria - classification
Bacteria - genetics
Birth
Childbirth & labor
Cross-Sectional Studies
Cultivation
Deoxyribonucleic acid
Disease
DNA
Feces
Female
Flora
Genetic aspects
Genetic polymorphisms
Genital microflora
Gynecology
Health aspects
Humans
Industrialized nations
Infant, Newborn
Intestinal microflora
Intestine
Intestines - microbiology
Labor
Lymphocytes
Medicine and Health Sciences
Metagenome
Microbiota
Microbiota (Symbiotic organisms)
Morbidity
Mortality
Neonates
Obstetrics
Polymerase chain reaction
Polymorphism
Polymorphism, Restriction Fragment Length
Pregnancy
Pregnant women
Premature birth
Premature Birth - etiology
Premature infants
Probiotics
Prospective Studies
Restriction fragment length polymorphism
Risk analysis
Risk Factors
RNA
Vagina
Vagina - microbiology
Vaginosis
Womens health
ISSN:1932-6203, 1932-6203
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Shrnutí:Preterm birth is a leading cause of perinatal morbidity and mortality. Studies using a cultivation method or molecular identification have shown that bacterial vaginosis is one of the risk factors for preterm birth. However, an association between preterm birth and intestinal microbiota has not been reported using molecular techniques, although the vaginal microbiota changes during pregnancy. Our aim here was to clarify the difference in intestinal and vaginal microbiota between women with preterm birth and women without preterm labor. 16S ribosomal ribonucleic acid genes were amplified from fecal and vaginal DNA by polymerase chain reaction. Using terminal restriction fragment length polymorphism (T-RFLP), we compared the levels of operational taxonomic units of both intestinal and vaginal flora among three groups: pregnant women who delivered term babies without preterm labor (non-PTL group) (n = 20), those who had preterm labor but delivered term babies (PTL group) (n = 11), and those who had preterm birth (PTB group) (n = 10). Significantly low levels of Clostridium subcluster XVIII, Clostridium cluster IV, Clostridium subcluster XIVa, and Bacteroides, and a significantly high level of Lactobacillales were observed in the intestinal microbiota in the PTB group compared with those in the non-PTL group. The levels of Clostridium subcluster XVIII and Clostridium subcluster XIVa in the PTB group were significantly lower than those in the PTL group, and these levels in the PTL group were significantly lower than those in non-PTL group. However, there were no significant differences in vaginal microbiota among the three groups. Intestinal microbiota in the PTB group was found to differ from that in the non-PTL group using the T-RFLP method.
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Competing Interests: TM and GS are employees of Toa Pharmaceutical Co., Ltd., whose company offered materials for sample collection and collected the samples. There are no patents, products in development or marketed products to declare. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials.
Conceived and designed the experiments: AS SY NY RY TM GS SS. Performed the experiments: AS SY NY RY TM GS SS. Analyzed the data: AS TM GS SS. Contributed reagents/materials/analysis tools: AS SY NY RY TM GS SS. Contributed to the writing of the manuscript: AS TM GS SS.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0111374