Reverse transcription recombinase polymerase amplification assay for rapid detection of canine associated rabies virus in Africa

Rabies is a neglected disease mostly affecting the developing world. Accurate and reliable diagnostic and surveillance data forms the foundation for the formulation and monitoring of control strategies. Although various sensitive and specific tests are available for detection of rabies virus, implem...

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Vydané v:	PloS one Ročník 14; číslo 7; s. e0219292
Hlavní autori:	Coertse, Jessica, Weyer, Jacqueline, Nel, Louis H., Markotter, Wanda
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States Public Library of Science 05.07.2019 Public Library of Science (PLoS)
Predmet:	Africa - epidemiology Amplification Animals Assaying Biological Assay Biology and life sciences Collaboration Developing countries Diagnosis Diagnostic systems Dog diseases Dogs - virology Editors Genetic aspects Health aspects Health sciences Infections Intelligence gathering Laboratories LDCs Lyssavirus Medicine and Health Sciences Methods Microbiological assay Molecular diagnostic techniques Nucleic Acid Amplification Techniques - methods Polymerase chain reaction Rabies Rabies - diagnosis Rabies - veterinary Rabies virus - genetics Rabies virus - pathogenicity Real-Time Polymerase Chain Reaction Recombinase Research and Analysis Methods Reverse transcriptase Reverse Transcriptase Polymerase Chain Reaction - methods Reverse transcription Ribonucleic acid RNA Sensitivity Sensitivity analysis Sensitivity and Specificity Surveillance Transcription (Genetics) Viral infections Virology Viruses Zoonoses Africa South Africa
ISSN:	1932-6203, 1932-6203
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Popis
Shrnutí:	Rabies is a neglected disease mostly affecting the developing world. Accurate and reliable diagnostic and surveillance data forms the foundation for the formulation and monitoring of control strategies. Although various sensitive and specific tests are available for detection of rabies virus, implementation of these tests in low-resource settings are challenging and remains limited. In this study, we describe the developed of a reverse transcription recombinase polymerase amplification assay for the detection of rabies virus. The analytical sensitivity of this assay was determined to be 562 RNA copies and was performed in 20 minutes. The diagnostic sensitivity of the RT-RPA was 100% for detection of rabies virus in field samples. In conclusion, the RT-RPA assay allowed for very quick and sensitive detection of rabies virus and could be adapted for use in low-source settings.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Competing Interests: The authors have declared that no competing interests exist.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0219292