Reverse transcription recombinase polymerase amplification assay for rapid detection of canine associated rabies virus in Africa
Rabies is a neglected disease mostly affecting the developing world. Accurate and reliable diagnostic and surveillance data forms the foundation for the formulation and monitoring of control strategies. Although various sensitive and specific tests are available for detection of rabies virus, implem...
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| Vydáno v: | PloS one Ročník 14; číslo 7; s. e0219292 |
|---|---|
| Hlavní autoři: | , , , |
| Médium: | Journal Article |
| Jazyk: | angličtina |
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United States
Public Library of Science
05.07.2019
Public Library of Science (PLoS) |
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| ISSN: | 1932-6203, 1932-6203 |
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| Abstract | Rabies is a neglected disease mostly affecting the developing world. Accurate and reliable diagnostic and surveillance data forms the foundation for the formulation and monitoring of control strategies. Although various sensitive and specific tests are available for detection of rabies virus, implementation of these tests in low-resource settings are challenging and remains limited. In this study, we describe the developed of a reverse transcription recombinase polymerase amplification assay for the detection of rabies virus. The analytical sensitivity of this assay was determined to be 562 RNA copies and was performed in 20 minutes. The diagnostic sensitivity of the RT-RPA was 100% for detection of rabies virus in field samples. In conclusion, the RT-RPA assay allowed for very quick and sensitive detection of rabies virus and could be adapted for use in low-source settings. |
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| AbstractList | Rabies is a neglected disease mostly affecting the developing world. Accurate and reliable diagnostic and surveillance data forms the foundation for the formulation and monitoring of control strategies. Although various sensitive and specific tests are available for detection of rabies virus, implementation of these tests in low-resource settings are challenging and remains limited. In this study, we describe the developed of a reverse transcription recombinase polymerase amplification assay for the detection of rabies virus. The analytical sensitivity of this assay was determined to be 562 RNA copies and was performed in 20 minutes. The diagnostic sensitivity of the RT-RPA was 100% for detection of rabies virus in field samples. In conclusion, the RT-RPA assay allowed for very quick and sensitive detection of rabies virus and could be adapted for use in low-source settings. Rabies is a neglected disease mostly affecting the developing world. Accurate and reliable diagnostic and surveillance data forms the foundation for the formulation and monitoring of control strategies. Although various sensitive and specific tests are available for detection of rabies virus, implementation of these tests in low-resource settings are challenging and remains limited. In this study, we describe the developed of a reverse transcription recombinase polymerase amplification assay for the detection of rabies virus. The analytical sensitivity of this assay was determined to be 562 RNA copies and was performed in 20 minutes. The diagnostic sensitivity of the RT-RPA was 100% for detection of rabies virus in field samples. In conclusion, the RT-RPA assay allowed for very quick and sensitive detection of rabies virus and could be adapted for use in low-source settings.Rabies is a neglected disease mostly affecting the developing world. Accurate and reliable diagnostic and surveillance data forms the foundation for the formulation and monitoring of control strategies. Although various sensitive and specific tests are available for detection of rabies virus, implementation of these tests in low-resource settings are challenging and remains limited. In this study, we describe the developed of a reverse transcription recombinase polymerase amplification assay for the detection of rabies virus. The analytical sensitivity of this assay was determined to be 562 RNA copies and was performed in 20 minutes. The diagnostic sensitivity of the RT-RPA was 100% for detection of rabies virus in field samples. In conclusion, the RT-RPA assay allowed for very quick and sensitive detection of rabies virus and could be adapted for use in low-source settings. |
| Audience | Academic |
| Author | Nel, Louis H. Markotter, Wanda Coertse, Jessica Weyer, Jacqueline |
| AuthorAffiliation | 2 The Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases, a Division of the National Health Laboratory Services, Sandringham, South Africa 1 Centre for Viral Zoonoses, Department of Medical Virology, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa 3 Centre for Viral Zoonoses, Department of Biochemistry, Genetics and Microbiology, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria, South Africa National Institute of Environmental Health Sciences (NIEHS), UNITED STATES |
| AuthorAffiliation_xml | – name: 1 Centre for Viral Zoonoses, Department of Medical Virology, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa – name: 2 The Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases, a Division of the National Health Laboratory Services, Sandringham, South Africa – name: National Institute of Environmental Health Sciences (NIEHS), UNITED STATES – name: 3 Centre for Viral Zoonoses, Department of Biochemistry, Genetics and Microbiology, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria, South Africa |
| Author_xml | – sequence: 1 givenname: Jessica orcidid: 0000-0002-8376-6508 surname: Coertse fullname: Coertse, Jessica – sequence: 2 givenname: Jacqueline surname: Weyer fullname: Weyer, Jacqueline – sequence: 3 givenname: Louis H. surname: Nel fullname: Nel, Louis H. – sequence: 4 givenname: Wanda surname: Markotter fullname: Markotter, Wanda |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/31276479$$D View this record in MEDLINE/PubMed |
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| CitedBy_id | crossref_primary_10_1007_s00253_020_10970_9 crossref_primary_10_1038_s41598_021_82479_8 crossref_primary_10_1002_bab_2207 crossref_primary_10_3389_fcimb_2024_1493492 crossref_primary_10_3201_eid2612_203592 crossref_primary_10_3390_v13040576 crossref_primary_10_4102_ojvr_v88i1_1911 crossref_primary_10_1016_j_mcp_2020_101580 crossref_primary_10_1016_j_heliyon_2023_e17148 crossref_primary_10_3389_fmolb_2020_582499 |
| Cites_doi | 10.1016/j.virusres.2011.09.010 10.7883/yoken.JJID.2009.187 10.1016/j.jviromet.2011.06.004 10.1186/s12917-017-0948-0 10.1371/journal.pntd.0003189 10.1016/j.jviromet.2011.05.007 10.1371/journal.pbio.0040204 10.1016/j.mcp.2014.11.005 10.1128/JCM.01256-10 10.1371/journal.pone.0071642 10.1016/j.trac.2017.10.015 10.1016/j.jviromet.2010.04.008 10.3201/eid1202.050812 10.20506/rst.37.2.2809 10.1016/j.ijid.2016.03.016 10.1016/j.jcv.2015.05.004 10.1007/s00705-018-3814-x 10.3390/tropicalmed2030030 10.1371/journal.pntd.0000530 10.1371/journal.pone.0058739 10.4102/jsava.v88i0.1397 10.4102/jsava.v86i1.1220 10.1186/s12985-017-0853-y 10.1371/journal.pntd.0004953 10.1373/clinchem.2015.245829 10.1371/journal.pntd.0003709 10.1371/journal.pone.0210018 10.3201/eid1212.060514 10.1371/journal.pntd.0001001 10.3201/eid1301.060810 10.1371/journal.pntd.0004776 10.1016/S0378-1135(02)00041-X 10.1128/mBio.00135-13 10.1371/journal.ppat.1006041 10.1186/s12917-018-1736-1 |
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| Copyright | COPYRIGHT 2019 Public Library of Science 2019 Coertse et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2019 Coertse et al 2019 Coertse et al |
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| References | M Mallewa (ref10) 2007; 13 T Lembo (ref13) 2006; 12 O Piepenburg (ref19) 2006; 4 RK Daher (ref43) 2015; 29 K Hampson (ref3) 2015; 9 E Robardet (ref36) 2011; 177 D David (ref17) 2002; 87 W Markotter (ref33) 2006; 12 A Abd El Wahed (ref24) 2015; 69 RK Daher (ref42) 2016; 62 A Coetzer (ref26) 2019; 14 A Coetzer (ref8) 2014; 8 (ref11) 2018 DS Boyle (ref22) 2013; 4 B Boldbaatar (ref40) 2009; 62 J Coertse (ref32) 2010; 48 DTS Hayman (ref39) 2011; 5 CE Rupprecht (ref12) 2018 M Fischer (ref35) 2012; 6 GK Amarasinghe (ref1) 2018; 163 AR Fooks (ref15) 2009; 3 J Coertse (ref28) 2016; 13 A Abd El Wahed (ref23) 2013; 8 W Markotter (ref2) 2018; 37 TP Scott (ref29) 2013; 8 P Patel (ref44) 2016; 10 L Ma (ref45) 2018; 14 E Eggerbauer (ref18) 2016; 10 W Muleya (ref37) 2012; 163 A Coetzer (ref9) 2017; 2 CE Rupprecht (ref14) 2018; 5 TA Hall (ref34) 1999 C Troupin (ref4) 2016; 12 S Wacharapluesadee (ref41) 2011; 175 ref6 Y Saitou (ref38) 2010; 168 ref5 V Duong (ref7) 2016; 46 J Li (ref20) 2019; 144 K Schlottau (ref25) 2017; 14 DJ Dean (ref30) 1996 W Markotter (ref16) 2015; 86 O Lobato IM (ref21) 2018; 98 A Coetzer (ref27) 2017; 88 J Coertse (ref31) 2015 |
| References_xml | – volume: 144 start-page: 31 year: 2019 ident: ref20 article-title: Review: a comprehensive summary of a decade development of the recombinase polymerase amplification. Analyst publication-title: Royal Society of Chemistry – volume: 163 start-page: 160 year: 2012 ident: ref37 article-title: Molecular epidemiology and a loop-mediated isothermal amplification method for diagnosis of infection with rabies virus in Zambia publication-title: Virus Res doi: 10.1016/j.virusres.2011.09.010 – volume: 62 start-page: 187 year: 2009 ident: ref40 article-title: Rapid detection of rabies virus by reverse transcription-loop-mediated isothermal amplification publication-title: Jpn J Infect Dis doi: 10.7883/yoken.JJID.2009.187 – volume: 177 start-page: 15 year: 2011 ident: ref36 article-title: International interlaboratory trials on rabies diagnosis: An overview of results and variation in reference diagnosis techniques (fluorescent antibody test, rabies tissue culture infection test, mouse inoculation test) and molecular biology techniques publication-title: J Virol Methods doi: 10.1016/j.jviromet.2011.06.004 – ident: ref5 – volume: 13 start-page: 37 year: 2016 ident: ref28 article-title: New isolations of the rabies-related Mokola virus from South Africa publication-title: BMC Vet Res doi: 10.1186/s12917-017-0948-0 – volume: 8 start-page: e3189 year: 2014 ident: ref8 article-title: Comparison of Biotinylated Monoclonal and Polyclonal Antibodies in an Evaluation of a Direct Rapid Immunohistochemical Test for the Routine Diagnosis of Rabies in Southern Africa publication-title: PLoS Negl Trop Dis doi: 10.1371/journal.pntd.0003189 – volume: 175 start-page: 278 year: 2011 ident: ref41 article-title: Comparative detection of rabies RNA by NASBA, real-time PCR and conventional PCR publication-title: J Virol Methods doi: 10.1016/j.jviromet.2011.05.007 – start-page: 88 year: 1996 ident: ref30 article-title: Laboratory techniques in rabies – volume: 4 start-page: e204 year: 2006 ident: ref19 article-title: DNA Detection Using Recombination Proteins. Haber J, editor publication-title: PLoS Biol doi: 10.1371/journal.pbio.0040204 – volume: 29 start-page: 116 year: 2015 ident: ref43 article-title: Influence of sequence mismatches on the specificity of recombinase polymerase amplification technology publication-title: Mol Cell Probes doi: 10.1016/j.mcp.2014.11.005 – volume: 48 start-page: 3949 year: 2010 ident: ref32 article-title: Improved PCR Methods for Detection of African Rabies and Rabies-Related Lyssaviruses publication-title: J Clin Microbiol doi: 10.1128/JCM.01256-10 – volume: 8 start-page: e71642 year: 2013 ident: ref23 article-title: A Portable Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of Foot-and-Mouth Disease Virus publication-title: PLoS One doi: 10.1371/journal.pone.0071642 – volume: 98 start-page: 19 year: 2018 ident: ref21 article-title: ’Sullivan CK. Recombinase polymerase amplification: Basics, applications and recent advances publication-title: TrAC Trends Anal Chem doi: 10.1016/j.trac.2017.10.015 – year: 2018 ident: ref12 article-title: Laboratory techniques in rabies – volume: 168 start-page: 13 year: 2010 ident: ref38 article-title: A method for simultaneous detection and identification of Brazilian dog- and vampire bat-related rabies virus by reverse transcription loop-mediated isothermal amplification assay publication-title: J Virol Methods doi: 10.1016/j.jviromet.2010.04.008 – year: 2018 ident: ref11 article-title: Manual of Diagnostic Tests and Vaccines for Terrestrial Animals – volume: 12 start-page: 310 year: 2006 ident: ref13 article-title: Evaluation of a direct, rapid immunohistochemical test for rabies diagnosis publication-title: Emerg Infect Dis doi: 10.3201/eid1202.050812 – volume: 37 start-page: 385 year: 2018 ident: ref2 article-title: Bat lyssaviruses publication-title: Rev Sci Tech Off Int Epiz doi: 10.20506/rst.37.2.2809 – volume: 46 start-page: 107 year: 2016 ident: ref7 article-title: Laboratory diagnostics in dog-mediated rabies: an overview of performance and a proposed strategy for various settings publication-title: Int J Infect Dis doi: 10.1016/j.ijid.2016.03.016 – volume: 69 start-page: 16 year: 2015 ident: ref24 article-title: Diagnostics-in-a-Suitcase: Development of a portable and rapid assay for the detection of the emerging avian influenza A (H7N9) virus publication-title: J Clin Virol doi: 10.1016/j.jcv.2015.05.004 – start-page: 95 year: 1999 ident: ref34 article-title: BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT publication-title: Nucleic Acids Symposium Series – volume: 163 start-page: 2283 year: 2018 ident: ref1 article-title: Taxonomy of the order Mononegavirales: update 2018 publication-title: Arch Virol doi: 10.1007/s00705-018-3814-x – volume: 2 start-page: 30 year: 2017 ident: ref9 article-title: Epidemiology of Rabies in Lesotho: The Importance of Routine Surveillance and Virus Characterization publication-title: Trop Med Infect Dis doi: 10.3390/tropicalmed2030030 – volume: 3 start-page: e530 year: 2009 ident: ref15 article-title: Emerging Technologies for the Detection of Rabies Virus: Challenges and Hopes in the 21st Century publication-title: PLoS Negl Trop Dis doi: 10.1371/journal.pntd.0000530 – volume: 6 start-page: 264 year: 2012 ident: ref35 article-title: Perspectives on molecular detection methods of lyssaviruses publication-title: Berl Munch Tierarztl Wochenschr – volume: 5 year: 2018 ident: ref14 article-title: Additional progress in the development and application of a direct, rapid immunohistochemical test for rabies diagnosis publication-title: Vet Sci – volume: 8 start-page: e58739 year: 2013 ident: ref29 article-title: Complete Genome and Molecular Epidemiological Data Infer the Maintenance of Rabies among Kudu (Tragelaphus strepsiceros) in Namibia publication-title: PLoS One doi: 10.1371/journal.pone.0058739 – volume: 88 start-page: 1 year: 2017 ident: ref27 article-title: Enhanced diagnosis of rabies and molecular evidence for the transboundary spread of the disease in Mozambique publication-title: J S Afr Vet Assoc doi: 10.4102/jsava.v88i0.1397 – volume: 86 start-page: 1 year: 2015 ident: ref16 article-title: Utility of forensic detection of rabies virus in decomposed exhumed dog carcasses publication-title: J S Afr Vet Assoc doi: 10.4102/jsava.v86i1.1220 – volume: 14 start-page: 184 year: 2017 ident: ref25 article-title: Development of molecular confirmation tools for swift and easy rabies diagnostics publication-title: Virol J. Virology Journal doi: 10.1186/s12985-017-0853-y – volume: 10 start-page: e0004953 year: 2016 ident: ref44 article-title: A Field-Deployable Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of the Chikungunya Virus publication-title: PLoS Negl Trop Dis doi: 10.1371/journal.pntd.0004953 – ident: ref6 – volume: 62 start-page: 947 year: 2016 ident: ref42 article-title: Recombinase Polymerase Amplification for Diagnostic Applications publication-title: Clin Chem doi: 10.1373/clinchem.2015.245829 – volume: 9 start-page: e0003709 year: 2015 ident: ref3 article-title: Estimating the Global Burden of Endemic Canine Rabies publication-title: PLoS Negl Trop Dis doi: 10.1371/journal.pntd.0003709 – volume: 14 start-page: e0210018 year: 2019 ident: ref26 article-title: Epidemiological aspects of the persistent transmission of rabies during an outbreak (2010–2017) in Harare, Zimbabwe publication-title: PLoS One doi: 10.1371/journal.pone.0210018 – start-page: 63 year: 2015 ident: ref31 article-title: Current Laboratory Techniques in Rabies Diagnosis, Research and Prevention – volume: 12 start-page: 1913 year: 2006 ident: ref33 article-title: Isolation of Lagos Bat Virus from Water Mongoose publication-title: Emerg Infect Dis doi: 10.3201/eid1212.060514 – volume: 5 start-page: e1001 year: 2011 ident: ref39 article-title: Evolutionary History of Rabies in Ghana publication-title: PLoS Negl Trop Dis doi: 10.1371/journal.pntd.0001001 – volume: 13 start-page: 136 year: 2007 ident: ref10 article-title: Rabies Encephalitis in Malaria-Endemic Area, Malawi, Africa publication-title: Emerg Infect Dis doi: 10.3201/eid1301.060810 – volume: 10 start-page: e0004776 year: 2016 ident: ref18 article-title: Evaluation of Six Commercially Available Rapid Immunochromatographic Tests for the Diagnosis of Rabies in Brain Material publication-title: PLoS Negl Trop Dis doi: 10.1371/journal.pntd.0004776 – volume: 87 start-page: 111 year: 2002 ident: ref17 article-title: Rabies virus detection by RT-PCR in decomposed naturally infected brains publication-title: Vet Microbiol doi: 10.1016/S0378-1135(02)00041-X – volume: 4 start-page: e00135 year: 2013 ident: ref22 article-title: Rapid Detection of HIV-1 Proviral DNA for Early Infant Diagnosis Using Recombinase Polymerase Amplification publication-title: MBio doi: 10.1128/mBio.00135-13 – volume: 12 start-page: e1006041 year: 2016 ident: ref4 article-title: Large-Scale Phylogenomic Analysis Reveals the Complex Evolutionary History of Rabies Virus in Multiple Carnivore Hosts publication-title: PLOS Pathog doi: 10.1371/journal.ppat.1006041 – volume: 14 start-page: 399 year: 2018 ident: ref45 article-title: Development and evaluation of a broadly reactive reverse transcription recombinase polymerase amplification assay for rapid detection of murine norovirus publication-title: BMC Vet Res. 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| Title | Reverse transcription recombinase polymerase amplification assay for rapid detection of canine associated rabies virus in Africa |
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