Reverse transcription recombinase polymerase amplification assay for rapid detection of canine associated rabies virus in Africa
Rabies is a neglected disease mostly affecting the developing world. Accurate and reliable diagnostic and surveillance data forms the foundation for the formulation and monitoring of control strategies. Although various sensitive and specific tests are available for detection of rabies virus, implem...
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| Published in: | PloS one Vol. 14; no. 7; p. e0219292 |
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| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
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Public Library of Science
05.07.2019
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| ISSN: | 1932-6203, 1932-6203 |
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| Abstract | Rabies is a neglected disease mostly affecting the developing world. Accurate and reliable diagnostic and surveillance data forms the foundation for the formulation and monitoring of control strategies. Although various sensitive and specific tests are available for detection of rabies virus, implementation of these tests in low-resource settings are challenging and remains limited. In this study, we describe the developed of a reverse transcription recombinase polymerase amplification assay for the detection of rabies virus. The analytical sensitivity of this assay was determined to be 562 RNA copies and was performed in 20 minutes. The diagnostic sensitivity of the RT-RPA was 100% for detection of rabies virus in field samples. In conclusion, the RT-RPA assay allowed for very quick and sensitive detection of rabies virus and could be adapted for use in low-source settings. |
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| AbstractList | Rabies is a neglected disease mostly affecting the developing world. Accurate and reliable diagnostic and surveillance data forms the foundation for the formulation and monitoring of control strategies. Although various sensitive and specific tests are available for detection of rabies virus, implementation of these tests in low-resource settings are challenging and remains limited. In this study, we describe the developed of a reverse transcription recombinase polymerase amplification assay for the detection of rabies virus. The analytical sensitivity of this assay was determined to be 562 RNA copies and was performed in 20 minutes. The diagnostic sensitivity of the RT-RPA was 100% for detection of rabies virus in field samples. In conclusion, the RT-RPA assay allowed for very quick and sensitive detection of rabies virus and could be adapted for use in low-source settings. Rabies is a neglected disease mostly affecting the developing world. Accurate and reliable diagnostic and surveillance data forms the foundation for the formulation and monitoring of control strategies. Although various sensitive and specific tests are available for detection of rabies virus, implementation of these tests in low-resource settings are challenging and remains limited. In this study, we describe the developed of a reverse transcription recombinase polymerase amplification assay for the detection of rabies virus. The analytical sensitivity of this assay was determined to be 562 RNA copies and was performed in 20 minutes. The diagnostic sensitivity of the RT-RPA was 100% for detection of rabies virus in field samples. In conclusion, the RT-RPA assay allowed for very quick and sensitive detection of rabies virus and could be adapted for use in low-source settings.Rabies is a neglected disease mostly affecting the developing world. Accurate and reliable diagnostic and surveillance data forms the foundation for the formulation and monitoring of control strategies. Although various sensitive and specific tests are available for detection of rabies virus, implementation of these tests in low-resource settings are challenging and remains limited. In this study, we describe the developed of a reverse transcription recombinase polymerase amplification assay for the detection of rabies virus. The analytical sensitivity of this assay was determined to be 562 RNA copies and was performed in 20 minutes. The diagnostic sensitivity of the RT-RPA was 100% for detection of rabies virus in field samples. In conclusion, the RT-RPA assay allowed for very quick and sensitive detection of rabies virus and could be adapted for use in low-source settings. |
| Audience | Academic |
| Author | Nel, Louis H. Markotter, Wanda Coertse, Jessica Weyer, Jacqueline |
| AuthorAffiliation | 2 The Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases, a Division of the National Health Laboratory Services, Sandringham, South Africa 1 Centre for Viral Zoonoses, Department of Medical Virology, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa 3 Centre for Viral Zoonoses, Department of Biochemistry, Genetics and Microbiology, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria, South Africa National Institute of Environmental Health Sciences (NIEHS), UNITED STATES |
| AuthorAffiliation_xml | – name: 1 Centre for Viral Zoonoses, Department of Medical Virology, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa – name: 2 The Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases, a Division of the National Health Laboratory Services, Sandringham, South Africa – name: National Institute of Environmental Health Sciences (NIEHS), UNITED STATES – name: 3 Centre for Viral Zoonoses, Department of Biochemistry, Genetics and Microbiology, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria, South Africa |
| Author_xml | – sequence: 1 givenname: Jessica orcidid: 0000-0002-8376-6508 surname: Coertse fullname: Coertse, Jessica – sequence: 2 givenname: Jacqueline surname: Weyer fullname: Weyer, Jacqueline – sequence: 3 givenname: Louis H. surname: Nel fullname: Nel, Louis H. – sequence: 4 givenname: Wanda surname: Markotter fullname: Markotter, Wanda |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/31276479$$D View this record in MEDLINE/PubMed |
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| CitedBy_id | crossref_primary_10_1007_s00253_020_10970_9 crossref_primary_10_1038_s41598_021_82479_8 crossref_primary_10_1002_bab_2207 crossref_primary_10_3389_fcimb_2024_1493492 crossref_primary_10_3201_eid2612_203592 crossref_primary_10_3390_v13040576 crossref_primary_10_4102_ojvr_v88i1_1911 crossref_primary_10_1016_j_mcp_2020_101580 crossref_primary_10_1016_j_heliyon_2023_e17148 crossref_primary_10_3389_fmolb_2020_582499 |
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| ContentType | Journal Article |
| Copyright | COPYRIGHT 2019 Public Library of Science 2019 Coertse et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2019 Coertse et al 2019 Coertse et al |
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| Title | Reverse transcription recombinase polymerase amplification assay for rapid detection of canine associated rabies virus in Africa |
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