Reverse transcription recombinase polymerase amplification assay for rapid detection of canine associated rabies virus in Africa

Rabies is a neglected disease mostly affecting the developing world. Accurate and reliable diagnostic and surveillance data forms the foundation for the formulation and monitoring of control strategies. Although various sensitive and specific tests are available for detection of rabies virus, implem...

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Bibliographic Details
Published in:PloS one Vol. 14; no. 7; p. e0219292
Main Authors: Coertse, Jessica, Weyer, Jacqueline, Nel, Louis H., Markotter, Wanda
Format: Journal Article
Language:English
Published: United States Public Library of Science 05.07.2019
Public Library of Science (PLoS)
Subjects:
Africa - epidemiology
Amplification
Animals
Assaying
Biological Assay
Biology and life sciences
Collaboration
Developing countries
Diagnosis
Diagnostic systems
Dog diseases
Dogs - virology
Editors
Genetic aspects
Health aspects
Health sciences
Infections
Intelligence gathering
Laboratories
LDCs
Lyssavirus
Medicine and Health Sciences
Methods
Microbiological assay
Molecular diagnostic techniques
Nucleic Acid Amplification Techniques - methods
Polymerase chain reaction
Rabies
Rabies - diagnosis
Rabies - veterinary
Rabies virus - genetics
Rabies virus - pathogenicity
Real-Time Polymerase Chain Reaction
Recombinase
Research and Analysis Methods
Reverse transcriptase
Reverse Transcriptase Polymerase Chain Reaction - methods
Reverse transcription
Ribonucleic acid
RNA
Sensitivity
Sensitivity analysis
Sensitivity and Specificity
Surveillance
Transcription (Genetics)
Viral infections
Virology
Viruses
Zoonoses
Africa
South Africa
ISSN:1932-6203, 1932-6203
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Description
Summary:Rabies is a neglected disease mostly affecting the developing world. Accurate and reliable diagnostic and surveillance data forms the foundation for the formulation and monitoring of control strategies. Although various sensitive and specific tests are available for detection of rabies virus, implementation of these tests in low-resource settings are challenging and remains limited. In this study, we describe the developed of a reverse transcription recombinase polymerase amplification assay for the detection of rabies virus. The analytical sensitivity of this assay was determined to be 562 RNA copies and was performed in 20 minutes. The diagnostic sensitivity of the RT-RPA was 100% for detection of rabies virus in field samples. In conclusion, the RT-RPA assay allowed for very quick and sensitive detection of rabies virus and could be adapted for use in low-source settings.
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Competing Interests: The authors have declared that no competing interests exist.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0219292