Prenylated flavonoid morusin protects against TNBS-induced colitis in rats

Morusin is a prenylated flavonoid isolated from the root bark of Morus alba. Many studies have shown the ability of flavonoids to act as anti-inflammatory agents. The aim of this study was to evaluate the effect of morusin on experimentally colitis induced by 2,4,6-trinitrobenzensulfonic acid in Wis...

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Bibliographic Details
Published in:PloS one Vol. 12; no. 8; p. e0182464
Main Authors: Vochyánová, Zora, Pokorná, Marie, Rotrekl, Dominik, Smékal, Václav, Fictum, Petr, Suchý, Pavel, Gajdziok, Jan, Šmejkal, Karel, Hošek, Jan
Format: Journal Article
Language:English
Published: United States Public Library of Science 10.08.2017
Public Library of Science (PLoS)
Subjects:
Animals
Anti-inflammatory agents
Antioxidants
Bark
Biology and Life Sciences
Biotechnology
Care and treatment
Catalase
Colitis
Colitis - chemically induced
Colitis - enzymology
Colitis - prevention & control
Colon
Colon - drug effects
Colon - enzymology
Colon - pathology
Colonoscopy
Damage
Dosage and administration
Enzymes
Flavonoids
Flavonoids - pharmacology
Gelatinase A
Gelatinase B
Inflammatory bowel disease
Inflammatory bowel diseases
Inflammatory diseases
Interleukin 1
Interleukins
Intestine
Male
Matrix Metalloproteinase 2 - metabolism
Matrix metalloproteinases
Medicine and Health Sciences
Molecular biology
Pharmaceutical sciences
Pharmacy
Prenylation
Rats
Rats, Wistar
Rodents
Signal transduction
Sulfasalazine
Superoxide dismutase
Transforming growth factor-b1
Transforming growth factors
Trinitrobenzenesulfonic Acid
Tumor necrosis factor-TNF
Veterinary medicine
Czech Republic
ISSN:1932-6203, 1932-6203
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Summary:Morusin is a prenylated flavonoid isolated from the root bark of Morus alba. Many studies have shown the ability of flavonoids to act as anti-inflammatory agents. The aim of this study was to evaluate the effect of morusin on experimentally colitis induced by 2,4,6-trinitrobenzensulfonic acid in Wistar rats and to compare it with sulfasalazine, a drug conventionally used in the treatment of inflammatory bowel disease. Morusin was administered by gavage at doses of 12.5, 25, or 50 mg/kg/day for five days. The colonic tissue was evaluated macroscopically, histologically, and by performing immunodetection and zymographic analysis to determine the levels of antioxidant enzymes [superoxide dismutase (SOD) and catalase (CAT)], interleukin (IL)-1β, and transforming growth factor (TGF)-β1 and the activities of matrix metalloproteinases (MMP) 2 and 9. The tissue damage scores were significantly reduced with increasing dose of morusin, however efficacy was not demonstrated at the highest dose. At the dose of 12.5 mg/kg, morusin exerted therapeutic effectivity similar to that of sulfasalazine (50 mg/kg). This was associated with significant reduction of TGF-β1 levels and MMP2 and MMP9 activities, and slight reduction of IL-1β. Our results suggest that morusin possesses therapeutic potential for the treatment of chronic inflammatory diseases.
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Competing Interests: The authors have declared that no competing interests exist.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0182464