A Streamlined DNA Tool for Global Identification of Heavily Exploited Coastal Shark Species (Genus Rhizoprionodon)

Obtaining accurate species-specific landings data is an essential step toward achieving sustainable shark fisheries. Globally distributed sharpnose sharks (genus Rhizoprionodon) exhibit life-history characteristics (rapid growth, early maturity, annual reproduction) that suggests that they could be...

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Bibliographic Details
Published in:PloS one Vol. 7; no. 4; p. e34797
Main Authors: Pinhal, Danillo, Shivji, Mahmood S., Nachtigall, Pedro G., Chapman, Demian D., Martins, Cesar
Format: Journal Article
Language:English
Published: United States Public Library of Science 09.04.2012
Public Library of Science (PLoS)
Subjects:
Animals
Biology
Body parts
Carcharhinidae
Carcharodon carcharias
Cetorhinidae
Cetorhinus maximus
Coastal fisheries
Deoxyribonucleic acid
DNA
DNA Primers - genetics
DNA, Ribosomal Spacer - genetics
Elasmobranchii
Endangered & extinct species
Fins
Fisheries
Genetics
Identification
International economic relations
Law enforcement
Life history
Marine conservation
Morphology
Multiplex Polymerase Chain Reaction - methods
Nucleotide sequence
Rhizoprionodon
Rhizoprionodon lalandii
Rhizoprionodon longurio
Rhizoprionodon oligolinx
Rhizoprionodon porosus
Ribosomal DNA
Sharks
Sharks - classification
Sharks - genetics
Species
Species Specificity
Sphyrna
Studies
Sustainability
Sustainable fisheries
Trends
Brazil
Florida
United States > US
India
ISSN:1932-6203, 1932-6203
Online Access:Get full text
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Summary:Obtaining accurate species-specific landings data is an essential step toward achieving sustainable shark fisheries. Globally distributed sharpnose sharks (genus Rhizoprionodon) exhibit life-history characteristics (rapid growth, early maturity, annual reproduction) that suggests that they could be fished in a sustainable manner assuming an investment in monitoring, assessment and careful management. However, obtaining species-specific landings data for sharpnose sharks is problematic because they are morphologically very similar to one another. Moreover, sharpnose sharks may also be confused with other small sharks (either small species or juveniles of large species) once they are processed (i.e., the head and fins are removed). Here we present a highly streamlined molecular genetics approach based on seven species-specific PCR primers in a multiplex format that can simultaneously discriminate body parts from the seven described sharpnose shark species commonly occurring in coastal fisheries worldwide. The species-specific primers are based on nucleotide sequence differences among species in the nuclear ribosomal internal transcribed spacer 2 locus (ITS2). This approach also distinguishes sharpnose sharks from a wide range of other sharks (52 species) and can therefore assist in the regulation of coastal shark fisheries around the world.
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Conceived and designed the experiments: DP MSS CM. Performed the experiments: DP PGN. Analyzed the data: DP MSS PGN DDC CM. Contributed reagents/materials/analysis tools: MSS CM. Wrote the paper: DP MSS. Critically edited and revised previous drafts of the manuscript: DP MSS DDC CM.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0034797