A Streamlined DNA Tool for Global Identification of Heavily Exploited Coastal Shark Species (Genus Rhizoprionodon)

Obtaining accurate species-specific landings data is an essential step toward achieving sustainable shark fisheries. Globally distributed sharpnose sharks (genus Rhizoprionodon) exhibit life-history characteristics (rapid growth, early maturity, annual reproduction) that suggests that they could be...

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Vydáno v:	PloS one Ročník 7; číslo 4; s. e34797
Hlavní autoři:	Pinhal, Danillo, Shivji, Mahmood S., Nachtigall, Pedro G., Chapman, Demian D., Martins, Cesar
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States Public Library of Science 09.04.2012 Public Library of Science (PLoS)
Témata:	Animals Biology Body parts Carcharhinidae Carcharodon carcharias Cetorhinidae Cetorhinus maximus Coastal fisheries Deoxyribonucleic acid DNA DNA Primers - genetics DNA, Ribosomal Spacer - genetics Elasmobranchii Endangered & extinct species Fins Fisheries Genetics Identification International economic relations Law enforcement Life history Marine conservation Morphology Multiplex Polymerase Chain Reaction - methods Nucleotide sequence Rhizoprionodon Rhizoprionodon lalandii Rhizoprionodon longurio Rhizoprionodon oligolinx Rhizoprionodon porosus Ribosomal DNA Sharks Sharks - classification Sharks - genetics Species Species Specificity Sphyrna Studies Sustainability Sustainable fisheries Trends Brazil Florida United States > US India
ISSN:	1932-6203, 1932-6203
On-line přístup:	Získat plný text
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Popis
Shrnutí:	Obtaining accurate species-specific landings data is an essential step toward achieving sustainable shark fisheries. Globally distributed sharpnose sharks (genus Rhizoprionodon) exhibit life-history characteristics (rapid growth, early maturity, annual reproduction) that suggests that they could be fished in a sustainable manner assuming an investment in monitoring, assessment and careful management. However, obtaining species-specific landings data for sharpnose sharks is problematic because they are morphologically very similar to one another. Moreover, sharpnose sharks may also be confused with other small sharks (either small species or juveniles of large species) once they are processed (i.e., the head and fins are removed). Here we present a highly streamlined molecular genetics approach based on seven species-specific PCR primers in a multiplex format that can simultaneously discriminate body parts from the seven described sharpnose shark species commonly occurring in coastal fisheries worldwide. The species-specific primers are based on nucleotide sequence differences among species in the nuclear ribosomal internal transcribed spacer 2 locus (ITS2). This approach also distinguishes sharpnose sharks from a wide range of other sharks (52 species) and can therefore assist in the regulation of coastal shark fisheries around the world.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Conceived and designed the experiments: DP MSS CM. Performed the experiments: DP PGN. Analyzed the data: DP MSS PGN DDC CM. Contributed reagents/materials/analysis tools: MSS CM. Wrote the paper: DP MSS. Critically edited and revised previous drafts of the manuscript: DP MSS DDC CM.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0034797