Analysis of the Gut Microbiota in the Old Order Amish and Its Relation to the Metabolic Syndrome

Obesity has been linked to the human gut microbiota; however, the contribution of gut bacterial species to the obese phenotype remains controversial because of conflicting results from studies in different populations. To explore the possible dysbiosis of gut microbiota in obesity and its metabolic...

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Published in:PloS one Vol. 7; no. 8; p. e43052
Main Authors: Zupancic, Margaret L., Cantarel, Brandi L., Liu, Zhenqiu, Drabek, Elliott F., Ryan, Kathleen A., Cirimotich, Shana, Jones, Cheron, Knight, Rob, Walters, William A., Knights, Daniel, Mongodin, Emmanuel F., Horenstein, Richard B., Mitchell, Braxton D., Steinle, Nanette, Snitker, Soren, Shuldiner, Alan R., Fraser, Claire M.
Format: Journal Article
Language:English
Published: United States Public Library of Science 15.08.2012
Public Library of Science (PLoS)
Subjects:
Adult
Amish
Analysis
Bacteria
Biochemistry
Biology
Body mass
Body mass index
Body size
Complications
Developmental biology
Diabetes
Dysbacteriosis
Endocrinology
Feces
Female
Gastrointestinal surgery
Gastrointestinal Tract - microbiology
Genomes
Humans
Immunology
Inflammation
Intestinal microflora
Lifestyles
Male
Medicine
Metabolic disorders
Metabolic syndrome
Metabolic Syndrome - genetics
Metabolism
Metagenome
Microbiota
Microbiota (Symbiotic organisms)
Middle Aged
Network analysis
Nutrition
Obesity
Obesity - genetics
Pennsylvania
Phenotype
Phenotypes
Population studies
Preventive medicine
Regression Analysis
RNA
RNA, Ribosomal, 16S - genetics
rRNA 16S
Sequence Analysis, DNA
Type 2 diabetes
Weight control
United States > US
Maryland
Colorado
Baltimore Maryland
ISSN:1932-6203, 1932-6203
Online Access:Get full text
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Summary:Obesity has been linked to the human gut microbiota; however, the contribution of gut bacterial species to the obese phenotype remains controversial because of conflicting results from studies in different populations. To explore the possible dysbiosis of gut microbiota in obesity and its metabolic complications, we studied men and women over a range of body mass indices from the Old Order Amish sect, a culturally homogeneous Caucasian population of Central European ancestry. We characterized the gut microbiota in 310 subjects by deep pyrosequencing of bar-coded PCR amplicons from the V1-V3 region of the 16S rRNA gene. Three communities of interacting bacteria were identified in the gut microbiota, analogous to previously identified gut enterotypes. Neither BMI nor any metabolic syndrome trait was associated with a particular gut community. Network analysis identified twenty-two bacterial species and four OTUs that were either positively or inversely correlated with metabolic syndrome traits, suggesting that certain members of the gut microbiota may play a role in these metabolic derangements.
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Competing Interests: The authors have declared that no competing interests exist.
Conceived and designed the experiments: CMF ARS NS RH SS. Performed the experiments: KAR SC CJ EM MLZ. Analyzed the data: BLC ZL EFD RK WAW BM DK. Wrote the paper: CMF ARS BLC EFD.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0043052