Cell Migration Is Regulated by AGE-RAGE Interaction in Human Oral Cancer Cells In Vitro

Advanced glycation end products (AGEs) are produced in an irreversible non-enzymatic reaction of carbohydrates and proteins. Patients with diabetes mellitus (DM) are known to have elevated AGE levels, which is viewed as a risk factor of diabetes-related complications. In a clinical setting, it has b...

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Vydané v:	PloS one Ročník 9; číslo 10; s. e110542
Hlavní autori:	Ko, Shun-Yao, Ko, Hshin-An, Shieh, Tzong-Ming, Chang, Weng-Cheng, Chen, Hong-I, Chang, Shu-Shing, Lin, I-Hsuan
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States Public Library of Science 16.10.2014 Public Library of Science (PLoS)
Predmet:	Adult Advanced glycosylation end products Age Alzheimer's disease Angiogenesis Biology and Life Sciences Cancer Carbohydrates Cell adhesion & migration Cell growth Cell Line, Tumor Cell migration Cell Movement - genetics Cell Proliferation - drug effects Cell Proliferation - genetics Complications Correlation analysis Diabetes Diabetes Complications - genetics Diabetes Complications - pathology Diabetes mellitus Diabetes Mellitus - genetics Diabetes Mellitus - pathology Diabetics Enzymes Experiments Female Flavonoids - administration & dosage Fluorides Gelatinase A Gelatinase B Gene expression Gene Expression Regulation, Neoplastic - drug effects Glycation End Products, Advanced - genetics Glyceraldehyde Glycosylation Health risks Humans Male Malignancy Matrix Metalloproteinase 2 - biosynthesis Matrix Metalloproteinase 9 - biosynthesis Medicine Melanoma Metastases Metastasis Mouth cancer Mouth Neoplasms - complications Mouth Neoplasms - genetics Mouth Neoplasms - pathology Neoplasm Metastasis Oral cancer Patients Penicillin Phosphorylation Proteins Receptor for Advanced Glycation End Products - antagonists & inhibitors Receptor for Advanced Glycation End Products - genetics Risk factors RNA, Small Interfering RNA-mediated interference Science Survival United States > US Taiwan
ISSN:	1932-6203, 1932-6203
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Popis
Shrnutí:	Advanced glycation end products (AGEs) are produced in an irreversible non-enzymatic reaction of carbohydrates and proteins. Patients with diabetes mellitus (DM) are known to have elevated AGE levels, which is viewed as a risk factor of diabetes-related complications. In a clinical setting, it has been shown that patients with oral cancer in conjunction with DM have a higher likelihood of cancer metastasis and lower cancer survival rates. AGE-RAGE (a receptor of AGEs) is also correlated with metastasis and angiogenesis. Recent studies have suggested that the malignancy of cancer may be enhanced by glyceraldehyde-derived AGEs; however, the underlying mechanism remains unclear. This study examined the apparently close correlation between AGE-RAGE and the malignancy of SAS oral cancer cell line. In this study, AGEs increased ERK phosphorylation, enhanced cell migration, and promoted the expression of RAGE, MMP2, and MMP9. Using PD98059, RAGE antibody, and RAGE RNAi to block RAGE pathway resulted in the inhibition of ERK phosphorylation. Cell migration, MMP2 and MMP9 expression were also reduced by this treatment. Our findings demonstrate the importance of AGE-RAGE with regard to the malignancy of oral cancer, and help to explain the poor prognosis of DM subjects with oral cancer.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Competing Interests: The authors have declared that no competing interests exist. Conceived and designed the experiments: SYK. Performed the experiments: HAK SSC IHL. Analyzed the data: SYK HAK. Contributed reagents/materials/analysis tools: SYK TMS WCC HIC. Wrote the paper: SYK.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0110542