Systemic Lupus Erythematosus and Vitamin D Deficiency Are Associated with Shorter Telomere Length among African Americans: A Case-Control Study

Systemic lupus erythematosus (SLE) is a chronic systemic autoimmune disease that disproportionately affects African American females. The causes of SLE are unknown but postulated to be a combination of genetic predisposition and environmental triggers. Vitamin D deficiency is one of the possible env...

Celý popis

Uložené v:

Podrobná bibliografia
Vydané v:	PloS one Ročník 8; číslo 5; s. e63725
Hlavní autori:	Hoffecker, Brett M., Raffield, Laura M., Kamen, Diane L., Nowling, Tamara K.
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States Public Library of Science 20.05.2013 Public Library of Science (PLoS)
Predmet:	25-Hydroxyvitamin D Adult African American women African Americans African Americans - genetics Aging Antibodies Autoimmune diseases Biology Biomarkers Case-Control Studies Chromosomes Chronic conditions Correlation Deoxyribonucleic acid Disease control DNA Enzyme-linked immunosorbent assay Female Females Humans Leukocytes (mononuclear) Lupus Lupus Erythematosus, Systemic - blood Lupus Erythematosus, Systemic - genetics Medical research Medicine Middle Aged Minority & ethnic groups Multiplexing Patients Peripheral blood mononuclear cells Population studies Radioimmunoassay Systemic lupus erythematosus Telomere - genetics Telomere Homeostasis Telomeres Vitamin D Vitamin D - blood Vitamin D deficiency Vitamin D Deficiency - blood Vitamin D Deficiency - genetics Vitamin deficiency United States > US South Carolina
ISSN:	1932-6203, 1932-6203
On-line prístup:	Získať plný text
Tagy:	Pridať tag Žiadne tagy, Buďte prvý, kto otaguje tento záznam!

Popis
Shrnutí:	Systemic lupus erythematosus (SLE) is a chronic systemic autoimmune disease that disproportionately affects African American females. The causes of SLE are unknown but postulated to be a combination of genetic predisposition and environmental triggers. Vitamin D deficiency is one of the possible environmental triggers. In this study we evaluated relationships between vitamin D status, cellular aging (telomere length) and anti-telomere antibodies among African American Gullah women with SLE. The study population included African American female SLE patients and unaffected controls from the Sea Island region of South Carolina. Serum 25-hydroxyvitamin D levels were measured using a nonchromatographic radioimmunoassay. Telomere length was measured in genomic DNA of peripheral blood mononuclear cells (PBMCs) by monochrome multiplex quantitative PCR. Anti-telomere antibody levels were measured by enzyme-linked immunosorbent assay (ELISA). Patients with SLE had significantly shorter telomeres and higher anti-telomere antibody titers compared to age- and gender-matched unaffected controls. There was a positive correlation between anti-telomere antibody levels and disease activity among patients and a significant correlation of shorter telomeres with lower 25-hydroxyvitamin D levels in both patients and controls. In follow-up examination of a subset of the patients, the patients who remained vitamin D deficient tended to have shorter telomeres than those patients whose 25-hydroxyvitamin D levels were repleted. Increasing 25-hydroxyvitamin D levels in African American patients with SLE may be beneficial in maintaining telomere length and preventing cellular aging. Moreover, anti-telomere antibody levels may be a promising biomarker of SLE status and disease activity.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Competing Interests: The authors have declared that no competing interests exist. These authors also contributed equally to this work. Conceived and designed the experiments: DLK TKN. Performed the experiments: BMH LMR TKN. Analyzed the data: BMH LMR DLK TKN. Contributed reagents/materials/analysis tools: DLK TKN. Wrote the paper: BMH LMR DLK TKN.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0063725