Meiotically Stable Natural Epialleles of Sadhu, a Novel Arabidopsis Retroposon

Epigenetic variation is a potential source of genomic and phenotypic variation among different individuals in a population, and among different varieties within a species. We used a two-tiered approach to identify naturally occurring epigenetic alleles in the flowering plant Arabidopsis: a primary s...

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Vydané v:PLoS genetics Ročník 2; číslo 3; s. e36
Hlavní autori: Rangwala, Sanjida H, Elumalai, Rangasamy, Vanier, Cheryl, Ozkan, Hakan, Galbraith, David W, Richards, Eric J
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States Public Library of Science 01.03.2006
Public Library of Science (PLoS)
Predmet:
Alleles
Arabidopsis
Arabidopsis (Thale Cress)
Arabidopsis - genetics
Arabidopsis Proteins
Centromere - ultrastructure
Cloning
Cytosine
Deoxyribonucleic acid
DNA
DNA Methylation
Epigenetics
Epistasis, Genetic
Experiments
Genes, Plant
Genetic Variation
Genetics
Genetics/Epigenetics
Genome, Plant
Genomes
Mutation
Plant Science
Polymorphism, Genetic
Retroelements - genetics
Species Specificity
ISSN:1553-7404, 1553-7390, 1553-7404
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Popis
Shrnutí:Epigenetic variation is a potential source of genomic and phenotypic variation among different individuals in a population, and among different varieties within a species. We used a two-tiered approach to identify naturally occurring epigenetic alleles in the flowering plant Arabidopsis: a primary screen for transcript level polymorphisms among three strains (Col, Cvi, Ler), followed by a secondary screen for epigenetic alleles. Here, we describe the identification of stable, meiotically transmissible epigenetic alleles that correspond to one member of a previously uncharacterized non-LTR retroposon family, which we have designated Sadhu. The pericentromeric At2g10410 element is highly expressed in strain Col, but silenced in Ler and 18 other strains surveyed. Transcription of this locus is inversely correlated with cytosine methylation and both the expression and DNA methylation states map in a Mendelian manner to stable cis-acting variation. The silent Ler allele can be converted by the epigenetic modifier mutation ddm1 to a meiotically stable expressing allele with an identical primary nucleotide sequence, demonstrating that the variation responsible for transcript level polymorphism among Arabidopsis strains is epigenetic. We extended our characterization of the Sadhu family members and show that different elements are subject to both genetic and epigenetic variation in natural populations. These findings support the view that an important component of natural variation in retroelements is epigenetic.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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a Current address: Operon Biotechnologies, Huntsville, Alabama, United States of America
b Current address: Department of Field Crops, Faculty of Agriculture, University of Cukurova, Adana, Turkey
ISSN:1553-7404
1553-7390
1553-7404
DOI:10.1371/journal.pgen.0020036