Seeing the wood for the trees: towards improved quantification of glial cells in central nervous system tissue

The following mini-review attempts to guide researchers in the quantification of fluorescently-labelled proteins within cultured thick or chromogenically-stained proteins within thin sections of brain tissue. It follows from our examination of the utility of Fiji ImageJ thresholding and binarization...

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Bibliographic Details
Published in:	Neural regeneration research Vol. 13; no. 9; pp. 1520 - 1523
Main Authors:	Healy, Sinéad, McMahon, Jill, FitzGerald, Una
Format:	Journal Article
Language:	English
Published:	India Wolters Kluwer India Pvt. Ltd 01.09.2018 Medknow Publications and Media Pvt. Ltd Medknow Publications & Media Pvt. Ltd Galway Neuroscience Centre, School of Natural Sciences, National University of Ireland, Galway, Ireland Medknow Publications & Media Pvt Ltd Wolters Kluwer Medknow Publications
Subjects:	Accuracy Algorithms Brain research Fluorescent proteins Medical research Neuroglia Neuroimaging organotypic brain slice culture; glial cell quantification; thresholding algorithms; Fiji ImageJ; BioVoxxel plug-in; stereology Performance evaluation Protein expression Proteins Quality Researchers Review Variance analysis Fiji glial cell quantification stereology BioVoxxel plug-in Fiji ImageJ organotypic brain slice culture thresholding algorithms
ISSN:	1673-5374, 1876-7958
Online Access:	Get full text
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