Asiatic acid induces colon cancer cell growth inhibition and apoptosis through mitochondrial death cascade

Cancer is one of the leading causes of death in the world. The triterpenoid compound asiatic acid derived from the tropical medicinal plant Centella asiatica displays cytotoxic activity on fibroblast cells and several other kinds of cells. The present work studies asiatic acid-mediated growth inhibi...

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Vydané v:	Biological & pharmaceutical bulletin Ročník 32; číslo 8; s. 1399
Hlavní autori:	Tang, Xue-Lian, Yang, Xin-Ying, Jung, Hyun-Ju, Kim, Sung-Yeon, Jung, Suk-Yul, Choi, Du-Young, Park, Won-Cheol, Park, Hyun
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Japan 01.08.2009
Predmet:	Animals Antineoplastic Agents, Phytogenic - chemistry Antineoplastic Agents, Phytogenic - isolation & purification Antineoplastic Agents, Phytogenic - pharmacology Apoptosis - drug effects Blotting, Western Caspase 3 - metabolism Caspase 9 - metabolism Cell Cycle - drug effects Cell Line, Tumor Cell Proliferation - drug effects Centella - chemistry Chromatin - metabolism Colonic Neoplasms - pathology Colorectal Neoplasms - pathology Cytochromes c - metabolism DNA Fragmentation - drug effects Flow Cytometry Humans Membrane Potential, Mitochondrial - drug effects Mice Mice, Inbred BALB C Microscopy, Confocal Microscopy, Fluorescence Mitochondria - drug effects Molecular Structure Pentacyclic Triterpenes Poly Adenosine Diphosphate Ribose - metabolism Stomach Neoplasms - pathology Triterpenes - chemistry Triterpenes - isolation & purification Triterpenes - pharmacology
ISSN:	1347-5215, 1347-5215
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Shrnutí:	Cancer is one of the leading causes of death in the world. The triterpenoid compound asiatic acid derived from the tropical medicinal plant Centella asiatica displays cytotoxic activity on fibroblast cells and several other kinds of cells. The present work studies asiatic acid-mediated growth inhibition of cancer cells and the underlying mechanism. Asiatic acid markedly inhibited cancer cell proliferation. Apoptosis of SW480 human colon cancer cells was induced by asiatic acid as shown by flow cytometry, DNA fragmentation and nuclear chromatin condensation experiments. Through increasing mitochondrial membrane permeability and cytochrome c release from mitochondria into cytosol, asiatic acid induced caspase-9 activity, which further activated caspase-3 and poly(ADP-ribose) polymerase cleavage resulting in irreversible apoptotic death in the tumor cells. Taken together, these results suggest that mitochondrial death apoptosis cascade plays very important roles in asiatic acid-induced cancer apoptosis.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1347-5215 1347-5215
DOI:	10.1248/bpb.32.1399