Monitoring of antibiotic-induced alterations in the human intestinal microflora and detection of probiotic strains by use of terminal restriction fragment length polymorphism

Terminal restriction fragment length polymorphism (T-RFLP) was investigated as a tool for monitoring the human intestinal microflora during antibiotic treatment and during ingestion of a probiotic product. Fecal samples from eight healthy volunteers were taken before, during, and after administratio...

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Veröffentlicht in:Applied and environmental microbiology Jg. 71; H. 1; S. 501
Hauptverfasser: Jernberg, Cecilia, Sullivan, Asa, Edlund, Charlotta, Jansson, Janet K
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 01.01.2005
Schlagworte:
Anti-Bacterial Agents - pharmacology
Bacteria - classification
Bacteria - genetics
Bacteria - growth & development
Bacteria - isolation & purification
Clindamycin - pharmacology
Colony Count, Microbial
DNA Fingerprinting - methods
DNA, Bacterial - analysis
Eubacterium - genetics
Eubacterium - isolation & purification
Humans
Intestines - drug effects
Intestines - microbiology
Lactobacillus - genetics
Lactobacillus - isolation & purification
Polymorphism, Restriction Fragment Length
Probiotics
ISSN:0099-2240
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Zusammenfassung:Terminal restriction fragment length polymorphism (T-RFLP) was investigated as a tool for monitoring the human intestinal microflora during antibiotic treatment and during ingestion of a probiotic product. Fecal samples from eight healthy volunteers were taken before, during, and after administration of clindamycin. During treatment, four subjects were given a probiotic, and four subjects were given a placebo. Changes in the microbial intestinal community composition and relative abundance of specific microbial populations in each subject were monitored by using viable counts and T-RFLP fingerprints. T-RFLP was also used to monitor specific bacterial populations that were either positively or negatively affected by clindamycin. Some dominant bacterial groups, such as Eubacterium spp., were easily monitored by T-RFLP, while they were hard to recover by cultivation. Furthermore, the two probiotic Lactobacillus strains were easily tracked by T-RFLP and were shown to be the dominant Lactobacillus community members in the intestinal microflora of subjects who received the probiotic.
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ISSN:0099-2240
DOI:10.1128/AEM.71.1.501-506.2005