Monitoring of antibiotic-induced alterations in the human intestinal microflora and detection of probiotic strains by use of terminal restriction fragment length polymorphism

Terminal restriction fragment length polymorphism (T-RFLP) was investigated as a tool for monitoring the human intestinal microflora during antibiotic treatment and during ingestion of a probiotic product. Fecal samples from eight healthy volunteers were taken before, during, and after administratio...

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Vydané v:	Applied and environmental microbiology Ročník 71; číslo 1; s. 501
Hlavní autori:	Jernberg, Cecilia, Sullivan, Asa, Edlund, Charlotta, Jansson, Janet K
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States 01.01.2005
Predmet:	Anti-Bacterial Agents - pharmacology Bacteria - classification Bacteria - genetics Bacteria - growth & development Bacteria - isolation & purification Clindamycin - pharmacology Colony Count, Microbial DNA Fingerprinting - methods DNA, Bacterial - analysis Eubacterium - genetics Eubacterium - isolation & purification Humans Intestines - drug effects Intestines - microbiology Lactobacillus - genetics Lactobacillus - isolation & purification Polymorphism, Restriction Fragment Length Probiotics
ISSN:	0099-2240
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Shrnutí:	Terminal restriction fragment length polymorphism (T-RFLP) was investigated as a tool for monitoring the human intestinal microflora during antibiotic treatment and during ingestion of a probiotic product. Fecal samples from eight healthy volunteers were taken before, during, and after administration of clindamycin. During treatment, four subjects were given a probiotic, and four subjects were given a placebo. Changes in the microbial intestinal community composition and relative abundance of specific microbial populations in each subject were monitored by using viable counts and T-RFLP fingerprints. T-RFLP was also used to monitor specific bacterial populations that were either positively or negatively affected by clindamycin. Some dominant bacterial groups, such as Eubacterium spp., were easily monitored by T-RFLP, while they were hard to recover by cultivation. Furthermore, the two probiotic Lactobacillus strains were easily tracked by T-RFLP and were shown to be the dominant Lactobacillus community members in the intestinal microflora of subjects who received the probiotic.
Bibliografia:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Undefined-1 ObjectType-Feature-3 content type line 23
ISSN:	0099-2240
DOI:	10.1128/AEM.71.1.501-506.2005