NIN-like protein 8 is a master regulator of nitrate-promoted seed germination in Arabidopsis

Seeds respond to multiple different environmental stimuli that regulate germination. Nitrate stimulates germination in many plants but how it does so remains unclear. Here we show that the Arabidopsis NIN-like protein 8 (NLP8) is essential for nitrate-promoted seed germination. Seed germination in n...

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Published in:Nature Communications Vol. 7; no. 7; pp. 13179 - 11
Main Authors: 矢野 亮一, Yan Dawei, Easwaran Vanathy, Chau Vivian, Okamoto Masanori, Ierullo Matthew, Kimura Mitsuhiro, Endo Akira, Yano Ryoichi, Pasha Asher, Gong Yunchen, Bi Yong-Mei, Provart Nicolas, Guttman David, Krapp Anne, Rothstein Steven J., Nambara Eiji
Format: Journal Article
Language:English
Published: London Nature Publishing Group 12.10.2016
Nature Publishing Group UK
Nature Portfolio
Subjects:
38
38/15
38/91
631/449/1741
631/449/2653
631/449/2675
Abscisic acid
Abscisic Acid - metabolism
Abscisic Acid - pharmacology
Arabidopsis - drug effects
Arabidopsis - genetics
Arabidopsis - growth & development
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Chemical and Process Engineering
Conserved Sequence
Cytochrome P-450 Enzyme System - genetics
Cytochrome P-450 Enzyme System - metabolism
Engineering Sciences
Enzymes
Food engineering
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Germination - drug effects
Germination - genetics
Humanities and Social Sciences
Life Sciences
multidisciplinary
Nitrates
Nitrates - metabolism
Nitrates - pharmacology
Nitrogen
Plant Growth Regulators - metabolism
Plant Growth Regulators - pharmacology
Plant Proteins
Promoter Regions, Genetic
Protein Isoforms - genetics
Protein Isoforms - metabolism
Proteins
Science
Science (multidisciplinary)
Seed germination
Seeds
Seeds - drug effects
Seeds - genetics
Seeds - growth & development
Seeds - metabolism
Transcription Factors - genetics
Transcription Factors - metabolism
Vegetal Biology
Toronto Ontario Canada
Canada
Japan
ISSN:2041-1723, 2041-1723
Online Access:Get full text
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Summary:Seeds respond to multiple different environmental stimuli that regulate germination. Nitrate stimulates germination in many plants but how it does so remains unclear. Here we show that the Arabidopsis NIN-like protein 8 (NLP8) is essential for nitrate-promoted seed germination. Seed germination in nlp8 loss-of-function mutants does not respond to nitrate. NLP8 functions even in a nitrate reductase-deficient mutant background, and the requirement for NLP8 is conserved among Arabidopsis accessions. NLP8 reduces abscisic acid levels in a nitrate-dependent manner and directly binds to the promoter of CYP707A2, encoding an abscisic acid catabolic enzyme. Genetic analysis shows that NLP8-mediated promotion of seed germination by nitrate requires CYP707A2. Finally, we show that NLP8 localizes to nuclei and unlike NLP7, does not appear to be activated by nitrate-dependent nuclear retention of NLP7, suggesting that seeds have a unique mechanism for nitrate signalling.
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Present address: Department of Life Science and Biotechnology, The Faculty of Life and Environmental Science, Shimane University 1060, Nishikawatsu, Matsue-shi, Shimane 690-8504, Japan
Present address: The National Institute of Agrobiological Sciences (NIAS), 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan
ISSN:2041-1723
2041-1723
DOI:10.1038/ncomms13179