SARS-CoV-2 productively infects human brain microvascular endothelial cells

Background The emergence of the novel, pathogenic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global health emergency. SARS-CoV-2 is highly contagious and has a high mortality rate in severe patients. However, there is very limited information on the effect of SARS-CoV-...

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Vydáno v:Journal of neuroinflammation Ročník 19; číslo 1; s. 149 - 17
Hlavní autoři: Yang, Rui-Cheng, Huang, Kun, Zhang, Hui-Peng, Li, Liang, Zhang, Yu-Fei, Tan, Chen, Chen, Huan-Chun, Jin, Mei-Lin, Wang, Xiang-Ru
Médium: Journal Article
Jazyk:angličtina
Vydáno: London BioMed Central 15.06.2022
BioMed Central Ltd
Springer Nature B.V
BMC
Témata:
Amino acids
Analysis
Annotations
Biomedical and Life Sciences
Biomedicine
Blood-brain barrier
Brain
Care and treatment
Cell adhesion & migration
Chemokines
Coronaviruses
COVID-19
Cytokines
Diagnosis
Endothelial cells
Endothelium
Experiments
Genes
Genomes
Health aspects
Human brain microvascular endothelial cells
Immune response
Immunofluorescence
Immunology
Infections
Inflammatory response
Laboratory animals
Measurement
Membrane permeability
Microvasculature
Neurobiology
Neurologic manifestations of general diseases
Neurology
Neuropathogenesis
Neurosciences
Permeability
Phenotypes
Proteins
Public health
Risk factors
SARS-CoV-2
Severe acute respiratory syndrome coronavirus 2
Transcriptomics
Viruses
Western blotting
China
Beijing China
United States > US
Inflammatory response
SARS-CoV-2
Permeability
Human brain microvascular endothelial cells
Blood–brain barrier
ISSN:1742-2094, 1742-2094
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Popis
Shrnutí:Background The emergence of the novel, pathogenic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global health emergency. SARS-CoV-2 is highly contagious and has a high mortality rate in severe patients. However, there is very limited information on the effect of SARS-CoV-2 infection on the integrity of the blood–brain barrier (BBB). Methods RNA-sequencing profiling was performed to analyze the transcriptomic changes in human brain microvascular endothelial cells (hBMECs) after SARS-CoV-2 infection. Bioinformatic tools were used for differential analysis. Immunofluorescence, real-time quantitative PCR, and Western blotting analysis were used to explore biological phenotypes. Results A total of 927 differentially expressed genes were identified, 610 of which were significantly upregulated while the remaining 317 were downregulated. We verified the significant induction of cytokines, chemokines, and adhesion molecules in hBMECs by SARS-CoV-2, suggesting an activation of the vascular endothelium in brain. Moreover, we demonstrated that SARS-CoV-2 infection could increase the BBB permeability, by downregulating as well as remodeling the intercellular tight junction proteins. Conclusions Our findings demonstrated that SARS-CoV-2 infection can cause BBB dysfunction, providing novel insights into the understanding of SARS-CoV-2 neuropathogenesis. Moreover, this finding shall constitute a new approach for future prevention and treatment of SARS-CoV-2-induced CNS infection.
Bibliografie:ObjectType-Article-1
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ISSN:1742-2094
1742-2094
DOI:10.1186/s12974-022-02514-x