A live-cell imaging system for visualizing the transport of Marburg virus nucleocapsid-like structures

Background Live-cell imaging is a powerful tool for visualization of the spatio-temporal dynamics of moving signals in living cells. Although this technique can be utilized to visualize nucleocapsid transport in Marburg virus (MARV)- or Ebola virus-infected cells, the experiments require biosafety l...

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Vydáno v:	Virology journal Ročník 16; číslo 1; s. 159
Hlavní autoři:	Takamatsu, Yuki, Dolnik, Olga, Noda, Takeshi, Becker, Stephan
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London BioMed Central 19.12.2019 BioMed Central Ltd Springer Nature B.V BMC
Témata:	Actin Actin polymerization Antiviral agents Biological Transport Biomedical and Life Sciences Biomedicine Biosafety Cell culture Cell Line Cytoskeleton Drug development Drug Evaluation, Preclinical - methods Ebola virus Emerging viruses Fatalities Fluorescence Genomes Hepatocytes - virology Humans image analysis Image Processing, Computer-Assisted - methods Infections Infectious diseases Intravital Microscopy - methods Laboratories Live-cell imaging Marburg disease Marburg marburgvirus Marburg virus Marburg virus disease Marburgvirus Marburgvirus - growth & development Microscopy Microscopy, Fluorescence - methods Muscle proteins nucleocapsid Nucleocapsid - metabolism Nucleocapsid-like structures Nucleocapsids physiological transport Plasmids Polymerization Proteins Staining and Labeling - methods Velocity Viral proteins Viral Proteins - analysis Virology Viruses Germany Central Africa Fiji Live-cell imaging Nucleocapsid-like structures Actin polymerization Marburg virus
ISSN:	1743-422X, 1743-422X
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Popis
Shrnutí:	Background Live-cell imaging is a powerful tool for visualization of the spatio-temporal dynamics of moving signals in living cells. Although this technique can be utilized to visualize nucleocapsid transport in Marburg virus (MARV)- or Ebola virus-infected cells, the experiments require biosafety level-4 (BSL-4) laboratories, which are restricted to trained and authorized individuals. Methods To overcome this limitation, we developed a live-cell imaging system to visualize MARV nucleocapsid-like structures using fluorescence-conjugated viral proteins, which can be conducted outside BSL-4 laboratories. Results Our experiments revealed that nucleocapsid-like structures have similar transport characteristics to those of nucleocapsids observed in MARV-infected cells, both of which are mediated by actin polymerization. Conclusions We developed a non-infectious live cell imaging system to visualize intracellular transport of MARV nucleocapsid-like structures. This system provides a safe platform to evaluate antiviral drugs that inhibit MARV nucleocapsid transport.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	1743-422X 1743-422X
DOI:	10.1186/s12985-019-1267-9