A live-cell imaging system for visualizing the transport of Marburg virus nucleocapsid-like structures

Background Live-cell imaging is a powerful tool for visualization of the spatio-temporal dynamics of moving signals in living cells. Although this technique can be utilized to visualize nucleocapsid transport in Marburg virus (MARV)- or Ebola virus-infected cells, the experiments require biosafety l...

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Veröffentlicht in:Virology journal Jg. 16; H. 1; S. 159
Hauptverfasser: Takamatsu, Yuki, Dolnik, Olga, Noda, Takeshi, Becker, Stephan
Format: Journal Article
Sprache:Englisch
Veröffentlicht: London BioMed Central 19.12.2019
BioMed Central Ltd
Springer Nature B.V
BMC
Schlagworte:
Actin
Actin polymerization
Antiviral agents
Biological Transport
Biomedical and Life Sciences
Biomedicine
Biosafety
Cell culture
Cell Line
Cytoskeleton
Drug development
Drug Evaluation, Preclinical - methods
Ebola virus
Emerging viruses
Fatalities
Fluorescence
Genomes
Hepatocytes - virology
Humans
image analysis
Image Processing, Computer-Assisted - methods
Infections
Infectious diseases
Intravital Microscopy - methods
Laboratories
Live-cell imaging
Marburg disease
Marburg marburgvirus
Marburg virus
Marburg virus disease
Marburgvirus
Marburgvirus - growth & development
Microscopy
Microscopy, Fluorescence - methods
Muscle proteins
nucleocapsid
Nucleocapsid - metabolism
Nucleocapsid-like structures
Nucleocapsids
physiological transport
Plasmids
Polymerization
Proteins
Staining and Labeling - methods
Velocity
Viral proteins
Viral Proteins - analysis
Virology
Viruses
Germany
Central Africa
Fiji
Live-cell imaging
Nucleocapsid-like structures
Actin polymerization
Marburg virus
ISSN:1743-422X, 1743-422X
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Zusammenfassung:Background Live-cell imaging is a powerful tool for visualization of the spatio-temporal dynamics of moving signals in living cells. Although this technique can be utilized to visualize nucleocapsid transport in Marburg virus (MARV)- or Ebola virus-infected cells, the experiments require biosafety level-4 (BSL-4) laboratories, which are restricted to trained and authorized individuals. Methods To overcome this limitation, we developed a live-cell imaging system to visualize MARV nucleocapsid-like structures using fluorescence-conjugated viral proteins, which can be conducted outside BSL-4 laboratories. Results Our experiments revealed that nucleocapsid-like structures have similar transport characteristics to those of nucleocapsids observed in MARV-infected cells, both of which are mediated by actin polymerization. Conclusions We developed a non-infectious live cell imaging system to visualize intracellular transport of MARV nucleocapsid-like structures. This system provides a safe platform to evaluate antiviral drugs that inhibit MARV nucleocapsid transport.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1743-422X
1743-422X
DOI:10.1186/s12985-019-1267-9