Computational design of novel Cas9 PAM-interacting domains using evolution-based modelling and structural quality assessment

We present here an approach to protein design that combines (i) scarce functional information such as experimental data (ii) evolutionary information learned from a natural sequence variants and (iii) physics-grounded modeling. Using a Restricted Boltzmann Machine (RBM), we learn a sequence model of...

Full description

Saved in:

Bibliographic Details
Published in:	PLoS computational biology Vol. 19; no. 11; p. e1011621
Main Authors:	Malbranke, Cyril, Rostain, William, Depardieu, Florence, Cocco, Simona, Monasson, Rémi, Bikard, David
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 01.11.2023 PLOS Public Library of Science (PLoS)
Subjects:	Amino acid sequence Amino acids Analysis Biochemistry, Molecular Biology Biology and Life Sciences Boltzmann constant Information sources Life Sciences Ligands Machine learning Methods Modelling Mutation Physics Proteins Quality assessment Quality control Quantitative Methods Representations Research and Analysis Methods Structural Biology Sucrose France
ISSN:	1553-7358, 1553-734X, 1553-7358
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Be the first to leave a comment!