WRKY1 represses the WHIRLY1 transcription factor to positively regulate plant defense against geminivirus infection

Geminiviruses constitute the largest group of known plant viruses and cause devastating diseases and economic losses in many crops worldwide. Due to limited naturally occurring resistance genes, understanding plant antiviral defense against geminiviruses is critical for finding host factors of gemin...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:PLoS pathogens Jg. 19; H. 4; S. e1011319
Hauptverfasser: Sun, Shaoshuang, Li, Shupeng, Zhou, Xueping, Yang, Xiuling
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States Public Library of Science 01.04.2023
Public Library of Science (PLoS)
Schlagworte:
Analysis
Antiviral agents
Antiviral drugs
Begomovirus - metabolism
Biology and Life Sciences
Calmodulin
Calmodulin - metabolism
Cloning
Defense industry
Defense mechanisms
Development strategies
DNA methylation
Dosage and administration
Economic impact
Electrophoresis
Geminiviridae
Geminiviridae - genetics
Geminiviridae - metabolism
Gene Expression Regulation
Health aspects
Infection
Infections
Kinases
Leaf-curl
Leaves
Medical research
Medicine, Experimental
Methods
Nicotiana
Plant diseases
Plant Diseases - genetics
Plant genetics
Plant virus diseases
Plant viruses
Plants
Plants (botany)
Prevention
Proteins
Research and Analysis Methods
Risk factors
RNA-mediated interference
Signal transduction
Tomatoes
Transcription factors
Transcription Factors - genetics
Transcription Factors - metabolism
Virus diseases
Viruses
Yellow leaf
China
ISSN:1553-7374, 1553-7366, 1553-7374
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Geminiviruses constitute the largest group of known plant viruses and cause devastating diseases and economic losses in many crops worldwide. Due to limited naturally occurring resistance genes, understanding plant antiviral defense against geminiviruses is critical for finding host factors of geminiviruses and development of strategies for geminivirus control. Here we identified NbWRKY1 as a positive regulator of plant defense against geminivirus infection. Using tomato yellow leaf curl China virus/tomato yellow leaf curl China betasatellite (TYLCCNV/TYLCCNB) as a representative geminivirus, we found that NbWRKY1 was upregulated in response to TYLCCNV/TYLCCNB infection. Overexpression of NbWRKY1 attenuated TYLCCNV/TYLCCNB infection, whereas knockdown of NbWRKY1 enhanced plant susceptibility to TYLCCNV/TYLCCNB. We further revealed that NbWRKY1 bound to the promoter of the NbWHIRLY1 (NbWhy1) transcription factor and inhibited the transcription of NbWhy1. Consistently, NbWhy1 negatively regulates plant response against TYLCCNV/TYLCCNB. Overexpression of NbWhy1 significantly accelerated TYLCCNV/TYLCCNB infection. Conversely, knockdown of NbWhy1 led to impaired geminivirus infection. Furthermore, we demonstrated that NbWhy1 interfered with the antiviral RNAi defense and disrupted the interaction between calmodulin 3 and calmodulin-binding transcription activator-3. Moreover, the NbWRKY1-NbWhy1 also confers plant antiviral response toward tomato yellow leaf curl virus infection. Taken together, our findings suggest that NbWRKY1 positively regulates plant defense to geminivirus infection by repressing NbWhy1. We propose that the NbWRKY1-NbWhy1 cascade could be further employed to control geminiviruses.
Bibliographie:new_version
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
The authors have declared that no competing interests exist.
ISSN:1553-7374
1553-7366
1553-7374
DOI:10.1371/journal.ppat.1011319