The T7-Primer Is a Source of Experimental Bias and Introduces Variability between Microarray Platforms

Eberwine(-like) amplification of mRNA adds distinct 6-10 bp nucleotide stretches to the 5' end of amplified RNA transcripts. Analysis of over six thousand microarrays reveals that probes containing motifs complementary to these stretches are associated with aberrantly high signals up to a hundr...

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Bibliographic Details
Published in:	PloS one Vol. 3; no. 4; p. e1980
Main Authors:	Kerkhoven, Ron M., Sie, Daoud, Nieuwland, Marja, Heimerikx, Mike, De Ronde, Jorma, Brugman, Wim, Velds, Arno
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 23.04.2008 Public Library of Science (PLoS)
Subjects:	Aberration Acids Antisense RNA Bacteriophage T7 - metabolism Base Sequence Bias Binding Sites Breast cancer Datasets DNA microarrays DNA Primers - genetics DNA Primers - metabolism DNA probes DNA, Intergenic - genetics DNA-Directed RNA Polymerases - metabolism Enzymes Gene expression Gene Expression Regulation Gene sequencing Genetics and Genomics/Bioinformatics Genetics and Genomics/Gene Expression Genomes Humans Hybridization Messenger RNA Molecular Biology/Bioinformatics Molecular Sequence Data Oligonucleotide Array Sequence Analysis Operon - genetics Platforms Probes Quality control Ribonucleic acid RNA RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Viral - genetics Studies Viral Proteins - metabolism Netherlands
ISSN:	1932-6203, 1932-6203
Online Access:	Get full text
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