Invasion of midgut epithelial cells by a persistently transmitted virus is mediated by sugar transporter 6 in its insect vector

Insect transmission is obligatory for persistently transmitted viruses because the vector insect is the only means of virus spread in nature. The insect midgut is the first major barrier limiting virus acquisition, but the mechanisms by which viruses are able to cross the cell membrane and then infe...

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Vydané v:	PLoS pathogens Ročník 14; číslo 7; s. e1007201
Hlavní autori:	Qin, Faliang, Liu, Wenwen, Wu, Nan, Zhang, Lu, Zhang, Zhongkai, Zhou, Xueping, Wang, Xifeng
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States Public Library of Science 01.07.2018 Public Library of Science (PLoS)
Predmet:	Agricultural biotechnology Animals Arthropods Biology Biology and Life Sciences Cytoplasm Dengue fever Disease transmission Encephalitis Epithelial cells Flowers & plants Grassy stunt Host-Parasite Interactions - physiology Infections Insect carriers of disease Insect Proteins - metabolism Insect Vectors - metabolism Insect Vectors - virology Insects Intestinal Mucosa - metabolism Intestinal Mucosa - virology Laboratories Medicine and Health Sciences Midgut Monosaccharide Transport Proteins - metabolism Mosquitoes Nucleocapsids Physiological aspects Plant diseases Proteins Research and Analysis Methods Rice Sugar Tenuivirus - metabolism Tenuivirus - pathogenicity Transmission efficiency Viral infections Virus Diseases - metabolism Virus Diseases - transmission Viruses China Beijing China United States > US
ISSN:	1553-7374, 1553-7366, 1553-7374
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Shrnutí:	Insect transmission is obligatory for persistently transmitted viruses because the vector insect is the only means of virus spread in nature. The insect midgut is the first major barrier limiting virus acquisition, but the mechanisms by which viruses are able to cross the cell membrane and then infect the midgut epithelial cells of the insect have not been elucidated completely. Here, we found that the outer capsid or nucleocapsid protein (NP) of three viruses can interact and colocalize with sugar transporter 6 that is highly expressed in the midgut of Laodelphax striatellus (LsST6). In contrast, LsST6 did not interact with the NP of rice grassy stunt virus, which cannot be transmitted by the same planthopper. LsST6 not only altered the cellular location of viral proteins and then colocalized with them in the cell membrane, but also mediated the entry of rice stripe virus (RSV) particles into Spodoptera frugiperda 9 (Sf9) cells that expressed the heterologous gene LsST6. We further showed that RSV particles initially bound to the cell membrane of midgut epithelial cells where it colocalized with LsST6, and then invaded the cytoplasm. When LsST6 expression was knocked down, viral titre, acquisition percentage and transmission efficiency of the treated insect decreased significantly, but virus replication was not affected. This work thus uncovered a strategy by which LsST6 mediates viral entry into midgut epithelial cells and leads to successful transmission by the insect vector.
Bibliografia:	new_version ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 The authors have declared that no competing interests exist.
ISSN:	1553-7374 1553-7366 1553-7374
DOI:	10.1371/journal.ppat.1007201