High-level Relatedness among Mycobacterium abscessus subsp. massiliense Strains from Widely Separated Outbreaks

Three recently sequenced strains isolated from patients during an outbreak of Mycobacterium abscessus subsp. massiliense infections at a cystic fibrosis center in the United States were compared with 6 strains from an outbreak at a cystic fibrosis center in the United Kingdom and worldwide strains....

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Veröffentlicht in:Emerging infectious diseases Jg. 20; H. 3; S. 364 - 371
Hauptverfasser: Tettelin, Hervé, Davidson, Rebecca M., Agrawal, Sonia, Aitken, Moira L., Shallom, Shamira, Hasan, Nabeeh A., Strong, Michael, Nogueira de Moura, Vinicius Calado, De Groote, Mary Ann, Duarte, Rafael S., Hine, Erin, Parankush, Sushma, Su, Qi, Daugherty, Sean C., Fraser, Claire M., Brown-Elliott, Barbara A., Wallace, Richard J., Holland, Steven M., Sampaio, Elizabeth P., Olivier, Kenneth N., Jackson, Mary, Zelazny, Adrian M.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States U.S. National Center for Infectious Diseases 01.03.2014
Centers for Disease Control and Prevention
Schlagworte:
bacteria
Brazil
Comparative analysis
Cystic fibrosis
Cystic Fibrosis - complications
Disease Outbreaks
Genes
Genome, Bacterial
Health aspects
Humans
Infection
Multilocus Sequence Typing
Mycobacterium - classification
Mycobacterium - genetics
Mycobacterium - isolation & purification
Mycobacterium abscessus
Mycobacterium abscessus subsp. massiliense
Mycobacterium Infections - complications
Mycobacterium Infections - diagnosis
Mycobacterium Infections - epidemiology
Mycobacterium Infections - microbiology
Phylogeny
Polymorphism, Single Nucleotide
relatedness
tuberculosis and other mycobacteria
United Kingdom
United States
Brazil
United Kingdom
Maryland
relatedness
bacteria
geographically distant outbreaks
United States
cystic fibrosis
tuberculosis and other mycobacteria
United Kingdom
outbreaks
strains
Mycobacterium abscessus subsp. massiliense
Mycobacterium abscessus
ISSN:1080-6040, 1080-6059, 1080-6059
Online-Zugang:Volltext
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Zusammenfassung:Three recently sequenced strains isolated from patients during an outbreak of Mycobacterium abscessus subsp. massiliense infections at a cystic fibrosis center in the United States were compared with 6 strains from an outbreak at a cystic fibrosis center in the United Kingdom and worldwide strains. Strains from the 2 cystic fibrosis outbreaks showed high-level relatedness with each other and major-level relatedness with strains that caused soft tissue infections during an epidemic in Brazil. We identified unique single-nucleotide polymorphisms in cystic fibrosis and soft tissue outbreak strains, separate single-nucleotide polymorphisms only in cystic fibrosis outbreak strains, and unique genomic traits for each subset of isolates. Our findings highlight the necessity of identifying M. abscessus to the subspecies level and screening all cystic fibrosis isolates for relatedness to these outbreak strains. We propose 2 diagnostic strategies that use partial sequencing of rpoB and secA1 genes and a multilocus sequence typing protocol.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1080-6040
1080-6059
1080-6059
DOI:10.3201/eid2003.131106