Molecular epidemiology of pneumococci obtained from Gambian children aged 2–29 months with invasive pneumococcal disease during a trial of a 9-valent pneumococcal conjugate vaccine

Background The study describes the molecular epidemiology of Streptococcus pneumoniae causing invasive disease in Gambian children Methods One hundred and thirty-two S. pneumoniae isolates were recovered from children aged 2–29 months during the course of a pneumococcal conjugate vaccine trial condu...

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Published in:BMC infectious diseases Vol. 8; no. 1; p. 81
Main Authors: Antonio, Martin, Dada-Adegbola, Hannah, Biney, Ekow, Awine, Tim, O'Callaghan, John, Pfluger, Valentin, Enwere, Godwin, Okoko, Brown, Oluwalana, Claire, Vaughan, Adeola, Zaman, Syed MA, Pluschke, Gerd, Greenwood, Brian M, Cutts, Felicity, Adegbola, Richard A
Format: Journal Article
Language:English
Published: London BioMed Central 11.06.2008
BioMed Central Ltd
BMC
Subjects:
Alleles
Child, Preschool
Clinical Trials as Topic
Diagnosis
Dosage and administration
Gambia - epidemiology
Genetic aspects
Genotype
Humans
Infant
Infectious Diseases
Internal Medicine
Medical Microbiology
Medicine
Medicine & Public Health
Microbial Sensitivity Tests
Molecular Epidemiology
Parasitology
Pneumococcal Infections - epidemiology
Pneumococcal Infections - immunology
Pneumococcal Infections - microbiology
Pneumococcal vaccine
Pneumococcal Vaccines - administration & dosage
Pneumococcal Vaccines - immunology
Pneumonia, Pneumococcal
Research Article
Risk factors
Serotyping
Streptococcus pneumoniae
Streptococcus pneumoniae - classification
Streptococcus pneumoniae - drug effects
Streptococcus pneumoniae - genetics
Streptococcus pneumoniae - isolation & purification
Tropical Medicine
Gambia
Pneumococcal Disease
Invasive Pneumococcal Disease
Clonal Complex
Pneumococcal Conjugate Vaccine
Capsular Polysaccharide
ISSN:1471-2334, 1471-2334
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Summary:Background The study describes the molecular epidemiology of Streptococcus pneumoniae causing invasive disease in Gambian children Methods One hundred and thirty-two S. pneumoniae isolates were recovered from children aged 2–29 months during the course of a pneumococcal conjugate vaccine trial conducted in The Gambia of which 131 were characterized by serotyping, antibiotic susceptibility, BOX-PCR and MLST. Results Twenty-nine different serotypes were identified; serotypes 14, 19A, 12F, 5, 23F, and 1 were common and accounted for 58.3% of all serotypes overall. MLST analysis showed 72 sequence types (STs) of which 46 are novel. eBURST analysis using the stringent 6/7 identical loci definition, grouped the isolates into 17 clonal complexes and 32 singletons. The population structure of the 8 serotype 1 isolates obtained from 4 vaccinated and 2 unvaccinated children were the same (ST 618) except that one (ST3336) of the isolates from an unvaccinated child had a novel ST which is a single locus variant of ST 618. Conclusion We provide the first background data on the genetic structure of S. pneumoniae causing IPD prior to PC7V use in The Gambia. This data will be important for assessing the impact of PC7V in post-vaccine surveillance from The Gambia.
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ISSN:1471-2334
1471-2334
DOI:10.1186/1471-2334-8-81