Optimization of the Solid-state Fermentation and Properties of a Polysaccharide from Paecilomyces cicadae (Miquel) Samson and Its Antioxidant Activities In Vitro

The culture conditions for the yield of a polysaccharide (PCPS) produced by Paecilomyces cicadae (Miquel) Samson on solid-state fermentation were investigated using response surface methodology (RSM). Plackett-Burman design (PBD) was applied to screen out significant factors, followed by the paths o...

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Published in:PloS one Vol. 9; no. 2; p. e87578
Main Authors: Ren, Xueyong, He, Liang, Cheng, Junwen, Chang, Jianmin
Format: Journal Article
Language:English
Published: United States Public Library of Science 03.02.2014
Public Library of Science (PLoS)
Subjects:
Alcohol
Analysis
Antioxidants
Antioxidants (Nutrients)
Antioxidants - metabolism
Antioxidants - pharmacology
Arabinose
Biology
Biphenyl Compounds - antagonists & inhibitors
Biphenyl Compounds - metabolism
Chemistry
Chromatography
Chromatography, Gel
Conformation
Design optimization
Engineering
Fermentation
Food
Forestry
Free radicals
Fungi
Hydroxyl Radical - antagonists & inhibitors
Hydroxyl Radical - metabolism
Hydroxyl radicals
Inoculum
Laboratories
Light scattering
Mannose
Materials science
Molecular weight
Monosaccharides
Optimization
Paecilomyces
Paecilomyces - metabolism
Pharmaceutical industry
Picrates - antagonists & inhibitors
Picrates - metabolism
Polysaccharides
Polysaccharides - metabolism
Polysaccharides - pharmacology
Regression Analysis
Relative humidity
Response surface methodology
Rhamnose
Saccharides
Size exclusion chromatography
Solid state
Solid state fermentation
Superoxide
Superoxides - antagonists & inhibitors
Superoxides - metabolism
Xylose
ISSN:1932-6203, 1932-6203
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Summary:The culture conditions for the yield of a polysaccharide (PCPS) produced by Paecilomyces cicadae (Miquel) Samson on solid-state fermentation were investigated using response surface methodology (RSM). Plackett-Burman design (PBD) was applied to screen out significant factors, followed by the paths of steepest ascent to move to the nearest region of maximum response. Then Box-Behnken design (BBD) was conducted to optimize the final levels of the culture conditions. After analyzing the regression equation and the response surface contour plots, relative humidity 56.07%, inoculum 13.51 mL/100 g and temperature 27.09°C were found to be the optimal key parameters for PCPS production. The maximum predicted yield of PCPS was 10.76 mg/g under the optimized conditions. The resulting PCPS (FPCPS) generated at optimal conditions was purified by chromatography column and found to be composed of mannose (43.2%), rhamnose (32.1%), xylose (14.5%) and arabinose (10.2%). Based on the size exclusion chromatography combined with multi-angle laser light scattering (SEC-MALLS) analysis, FPCPS adopted a Gaussian coil conformation in 0.1 M NaNO3 solution with 3.75 × 10(6) g/mol of the weight-average molar mass (Mw) and 41.1 nm of the root-mean square radius (Rg(2))z (1/2). Furthermore, both of the polysaccharides were revealed to have strong antioxidant activities by evaluating in DPPH radical, superoxide radicals and hydroxyl radical assay. These data suggest the polysaccharides of Paecilomyces cicadae (Miquel) Samson produced by solid-state fermentation could be explored as potential natural antioxidants.
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Competing Interests: All the authors have declared that no competing interests exist.
Conceived and designed the experiments: JMC XYR LH. Performed the experiments: XYR JWC LH. Analyzed the data: XYR LH JMC. Contributed reagents/materials/analysis tools: XYR JWC LH. Wrote the paper: XYR LH.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0087578