Mitochondria-targeted ratiometric fluorescent probe for real time monitoring of pH in living cells

Pyridinium functioned 7-hydroxy coumarin was presented as the first mitochondria-targeted ratiometric fluorescent probe CP for real time monitoring pH in living cells. Compared with commercially available mitochondrial trackers, CP possesses high specificity to mitochondria in living cells as well a...

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Veröffentlicht in:Biomaterials Jg. 53; S. 669 - 678
Hauptverfasser: Wu, Ming-Yu, Li, Kun, Liu, Yan-Hong, Yu, Kang-Kang, Xie, Yong-Mei, Zhou, Xue-Dong, Yu, Xiao-Qi
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Netherlands Elsevier Ltd 01.06.2015
Schlagworte:
7-Hydroxy coumarin
acidification
Advanced Basic Science
apoptosis
biocompatibility
Cells (biology)
Cellular
confocal microscopy
coumarin
Dentistry
Fluorescence
fluorescent dyes
Fluorescent Dyes - chemistry
HeLa Cells
Humans
Hydrogen-Ion Concentration
mitochondria
Mitochondria - chemistry
Mitochondria-targeted
Monitoring
Monitors
pH probe
Ratiometric
Real time
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
stress response
Surgical implants
7-Hydroxy coumarin
Mitochondria-targeted
Real time
Ratiometric
pH probe
ISSN:0142-9612, 1878-5905, 1878-5905
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Beschreibung
Zusammenfassung:Pyridinium functioned 7-hydroxy coumarin was presented as the first mitochondria-targeted ratiometric fluorescent probe CP for real time monitoring pH in living cells. Compared with commercially available mitochondrial trackers, CP possesses high specificity to mitochondria in living cells as well as good biocompatibility. Meanwhile, CP displays excellent pH sensitivity and anti-interference capability. Confocal image experiments confirm that CP can monitor mitochondrial pH changes associated with the mitochondrial acidification, cellular apoptosis and stress response efficiently in real time.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0142-9612
1878-5905
1878-5905
DOI:10.1016/j.biomaterials.2015.02.113