Molecular, serological and in vitro culture-based characterization of Bourbon virus, a newly described human pathogen of the genus Thogotovirus

•Full-genomic analyses indicate that BRBV is a distinct member of the genus Thogotovirus.•Serological characterization further indicates that BRBV is a distinct member of the genus Thogotovirus.•Growth of BRBV in cell culture suggests association with tick and mammalian hosts.•Preliminary evaluation...

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Veröffentlicht in:Journal of clinical virology Jg. 73; S. 127 - 132
Hauptverfasser: Lambert, Amy J., Velez, Jason O., Brault, Aaron C., Calvert, Amanda E., Bell-Sakyi, Lesley, Bosco-Lauth, Angela M., Staples, J. Erin, Kosoy, Olga I.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Netherlands Elsevier B.V 01.12.2015
Schlagworte:
Allergy and Immunology
Animals
Bourbon virus
Cell Line
Characterization
Chlorocebus aethiops
Disease Models, Animal
Genome, Viral
HeLa Cells
Humans
In vitro growth
Infectious Disease
Influenza, Human - immunology
Influenza, Human - virology
Ixodidae
Male
Mice
Molecular
Orthomyxovirus
Phylogeny
Serological
Thogotovirus
Thogotovirus - classification
Thogotovirus - genetics
Thogotovirus - isolation & purification
Vero Cells
Viral Load
ORF
Bourbon virus
HTS
MOI
IP
Molecular
PRNT
Characterization
In vitro growth
CDS
RT-PCR
Serological
IC
BRBV
NJ
DPI
Thogotovirus
PFU
open reading frame
intracranial
days post onset
multiplicity of infection
Centers for Disease Control and Prevention
plaque reduction neutralization test
high throughput sequencing
neighbor joining
intraperitoneal
plaque forming unit
reverse transcription polymerase chain reaction
ISSN:1386-6532, 1873-5967, 1873-5967
Online-Zugang:Volltext
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Zusammenfassung:•Full-genomic analyses indicate that BRBV is a distinct member of the genus Thogotovirus.•Serological characterization further indicates that BRBV is a distinct member of the genus Thogotovirus.•Growth of BRBV in cell culture suggests association with tick and mammalian hosts.•Preliminary evaluation of BRBV in CD-1 mice in the generation of polyclonal sera reveal that these mice are susceptible to BRBV infection, but not disease. In June of 2014, a previously healthy man from Kansas with a recent history of tick exposure died from complications related to an illness marked by fever, thrombocytopenia and leukopenia. An isolate was derived from the blood of this patient during the course of diagnostic testing. This isolate was subsequently identified as a novel orthomyxovirus of the genus Thogotovirus by next generation sequencing and was named Bourbon virus after the patient’s county of residence. To support research and diagnostic aims, we provide a basic description of Bourbon virus at both the molecular and serological levels. Furthermore, to preliminarily identify potential host and vector range associations we have characterized the growth kinetics of Bourbon virus in a variety of vertebrate and invertebrate cell lines. Bourbon virus was subjected to next generation-high throughput sequencing, phylogenetic, and basic structural protein analyses as well as 2-way plaque reduction neutralization assays. Also, we inoculated a variety of cell types with Bourbon virus and evaluated the growth kinetics by determining viral titers in the supernatants taken from infected cells over time. Bourbon virus possesses 24–82% identity at the amino acid sequence level and low serological cross-reactivity with other Thogotoviruses. In vitro growth kinetics reveal robust replication of Bourbon virus in mammalian and tick cells. Molecular and serological characterizations identify Bourbon virus as a novel member of the genus Thogotovirus. Results from cell culture analyses suggest an association between Bourbon virus and mammalian and tick hosts.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1386-6532
1873-5967
1873-5967
DOI:10.1016/j.jcv.2015.10.021