Quantitative Targeted Absolute Proteomics for Better Characterization of an In Vitro Human Blood–Brain Barrier Model Derived from Hematopoietic Stem Cells

We previously developed an in vitro model of the human blood–brain barrier (BBB) based on the use of endothelial cells derived from CD34+-hematopoietic stem cells and cultured with brain pericytes. The purpose of the present study was to provide information on the protein expression levels of the tr...

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Bibliographic Details
Published in:	Cells (Basel, Switzerland) Vol. 11; no. 24; p. 3963
Main Authors:	Dehouck, Marie-Pierre, Tachikawa, Masanori, Hoshi, Yutaro, Omori, Kotaro, Maurage, Claude-Alain, Strecker, Guillaume, Dehouck, Lucie, Boucau, Marie-Christine, Uchida, Yasuo, Gosselet, Fabien, Terasaki, Tetsuya, Karamanos, Yannis
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 08.12.2022 MDPI
Subjects:	Analysis ATP Binding Cassette Transporter, Subfamily G, Member 2 - metabolism Biochemistry, Molecular Biology Blood & organ donations Blood vessels Blood-brain barrier Blood-Brain Barrier - metabolism Brain brain microvessels Caveolin Caveolin-1 CD147 antigen CD34 antigen Chromatography Chromatography, Liquid Claudin-5 - metabolism Connexin 43 Endothelial cells Endothelial Cells - metabolism Genomics Hematopoietic stem cells Hematopoietic Stem Cells - metabolism human brain-like endothelial cells human in vitro models Humans LC-MS/MS-based protein quantification Life Sciences Liquid chromatography Mass spectrometry Mass spectroscopy MDR1 protein Membrane Transport Proteins - metabolism Neoplasm Proteins - metabolism Neurobiology Neurons and Cognition Pericytes Permeability Protein expression Protein folding Proteins Proteomics Proteomics - methods Stem cells Tandem Mass Spectrometry - methods Transplantation transporter France United States > US blood–brain barrier human in vitro models human brain-like endothelial cells LC-MS/MS-based protein quantification brain microvessels transporter LC-MS/MS-based protein quantiﬁcation human brain-like endothelial cell
ISSN:	2073-4409, 2073-4409
Online Access:	Get full text
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