Induction of integration-free human-induced pluripotent stem cells under serum- and feeder-free conditions

Human-induced pluripotent stem cells (hiPSCs) have shown great potential toward practical and scientific applications. We previously reported the generation of human dental pulp stem cells using non-integrating replication-defective Sendai virus (SeVdp) vector in feeder-free culture with serum-free...

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Published in:In vitro cellular & developmental biology. Animal Vol. 56; no. 1; pp. 85 - 95
Main Authors: Hamada, Atsuko, Akagi, Eri, Yamasaki, Sachiko, Nakatao, Hirotaka, Obayashi, Fumitaka, Ohtaka, Manami, Nishimura, Ken, Nakanishi, Mahito, Toratani, Shigeaki, Okamoto, Tetsuji
Format: Journal Article
Language:English
Published: New York Springer Science & Business Media LLC 01.01.2020
Springer US
Society for In Vitro Biology
Subjects:
Animal Genetics and Genomics
Biomedical and Life Sciences
Biopsy
Blood
blood serum
Cell Biology
Cell Culture
cell differentiation
Culture
Dental materials
Dental pulp
Developmental Biology
Differentiation (biology)
Human influences
humans
induced pluripotent stem cells
Interleukin 2
Invasiveness
Laminin
Leukocytes (mononuclear)
Life Sciences
microarray technology
Molecular modelling
mononuclear leukocytes
Murine respirovirus
Peripheral blood mononuclear cells
Pluripotency
Serum-free medium
STEM CELLS
Substrates
tooth pulp
Viruses
Peripheral blood mononuclear cells
hESF9 serum-free defined media
Human-induced pluripotent stem cells
Reprogramming efficiency
ISSN:1071-2690, 1543-706X, 1543-706X
Online Access:Get full text
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Summary:Human-induced pluripotent stem cells (hiPSCs) have shown great potential toward practical and scientific applications. We previously reported the generation of human dental pulp stem cells using non-integrating replication-defective Sendai virus (SeVdp) vector in feeder-free culture with serum-free medium hESF9. This study describes the generation of hiPSCs from peripheral blood mononuclear cells to increase the donor population, while reducing biopsy invasiveness. From 6-d-old primary culture of peripheral blood mononuclear cells (PBMCs) with IL-2, hiPSCs were established using SeVdp(KOSM)302L with recombinant Laminin-511 E8 fragments under serum-free condition. The established PBMC-derived hiPSCs showed pluripotency and differentiation ability both in vivo and in vitro. In addition, we evaluated microarray data from PBMC- and dental pulp-derived hiPSCs. These hiPSCs will be beneficial for characterizing the molecular mechanisms of cellular differentiation and may provide useful substrates for developing cellular therapeutics.
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ISSN:1071-2690
1543-706X
1543-706X
DOI:10.1007/s11626-019-00412-w