Nanopore sequencing technology, bioinformatics and applications

Rapid advances in nanopore technologies for sequencing single long DNA and RNA molecules have led to substantial improvements in accuracy, read length and throughput. These breakthroughs have required extensive development of experimental and bioinformatics methods to fully exploit nanopore long rea...

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Bibliographic Details
Published in:Nature biotechnology Vol. 39; no. 11; pp. 1348 - 1365
Main Authors: Wang, Yunhao, Zhao, Yue, Bollas, Audrey, Wang, Yuru, Au, Kin Fai
Format: Journal Article
Language:English
Published: New York Nature Publishing Group US 01.11.2021
Nature Publishing Group
Subjects:
631/114/2785
631/1647/48
631/61/514
Agriculture
Bioinformatics
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Computational Biology
DNA sequencing
Genomes
Genomics
High-Throughput Nucleotide Sequencing - methods
Life Sciences
Methods
Nanopore Sequencing
Nanopores
Nucleotide sequencing
Review Article
Sequence Analysis, DNA - methods
Technology
Transcription
Transcriptomes
United States
ISSN:1087-0156, 1546-1696, 1546-1696
Online Access:Get full text
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Summary:Rapid advances in nanopore technologies for sequencing single long DNA and RNA molecules have led to substantial improvements in accuracy, read length and throughput. These breakthroughs have required extensive development of experimental and bioinformatics methods to fully exploit nanopore long reads for investigations of genomes, transcriptomes, epigenomes and epitranscriptomes. Nanopore sequencing is being applied in genome assembly, full-length transcript detection and base modification detection and in more specialized areas, such as rapid clinical diagnoses and outbreak surveillance. Many opportunities remain for improving data quality and analytical approaches through the development of new nanopores, base-calling methods and experimental protocols tailored to particular applications. Au and colleagues outline the field of nanopore sequencing.
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K.F.A. designed the outline of the article. Yunhao Wang and A.B. collected information and prepared the materials for the ‘Technology development’ and ‘Data analysis’ sections. Y.Z. collected information and prepared the materials for the ‘Applications of nanopore sequencing’ section. K.F.A., Yunhao Wang, Y.Z. and A.B. wrote and revised the main text. Yuru Wang collected the references for the ‘Applications of nanopore sequencing’ section and prepared Fig. 1.
Author contributions
ISSN:1087-0156
1546-1696
1546-1696
DOI:10.1038/s41587-021-01108-x