Grafting on a Non-Transgenic Tolerant Tomato Variety Confers Resistance to the Infection of a Sw5-Breaking Strain of Tomato spotted wilt virus via RNA Silencing

RNA silencing controls endogenous gene expression and drives defensive reactions against invasive nucleic acids like viruses. In plants, it has been demonstrated that RNA silencing can be transmitted through grafting between scions and silenced rootstocks to attenuate virus and viroid accumulation i...

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Vydáno v:	PloS one Ročník 10; číslo 10; s. e0141319
Hlavní autoři:	Spanò, Roberta, Mascia, Tiziana, Kormelink, Richard, Gallitelli, Donato
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States Public Library of Science 23.10.2015 Public Library of Science (PLoS)
Témata:	Agriculture Chromosome Mapping Disease Resistance Dynamic tests EPS-2 Flowers & plants Gene expression Gene Expression Regulation, Plant Gene silencing Genome, Plant Genotype Genotypes Grafting Infections Inoculation Invasive plants Laboratorium voor Virologie Laboratory of Virology Lycopersicon esculentum - genetics Lycopersicon esculentum - virology Nucleic acids Plant diseases Plant Diseases - virology Plant viruses Plants, Genetically Modified Proteins Ribonucleic acid RNA RNA Interference RNA Transport RNA viruses RNA, Plant - metabolism RNA, Small Untranslated - metabolism RNA-mediated interference Rootstocks Scions Tobacco Tomatoes Tospovirus - physiology Transgenic plants Viruses Wilt Italy
ISSN:	1932-6203, 1932-6203
On-line přístup:	Získat plný text
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Popis
Shrnutí:	RNA silencing controls endogenous gene expression and drives defensive reactions against invasive nucleic acids like viruses. In plants, it has been demonstrated that RNA silencing can be transmitted through grafting between scions and silenced rootstocks to attenuate virus and viroid accumulation in the scions. This has been obtained mostly using transgenic plants, which may be a drawback in current agriculture. In the present study, we examined the dynamics of infection of a resistance-breaking strain of Tomato spotted wilt virus (RB-TSWV) through the graft between an old Apulian (southern Italy) tomato variety, denoted Sl-Ma, used as a rootstock and commercial tomato varieties used as scions. In tests with non-grafted plants, Sl-Ma showed resistance to the RB-TSWV infection as viral RNA accumulated at low levels and plants recovered from disease symptoms by 21 days post inoculation. The resistance trait was transmitted to the otherwise highly susceptible tomato genotypes grafted onto Sl-Ma. The results from the analysis of small RNAs hallmark genes involved in RNA silencing and virus-induced gene silencing suggest that RNA silencing is involved in the resistance showed by Sl-Ma against RB-TSWV and in scions grafted on this rootstock. The results from self-grafted susceptible tomato varieties suggest also that RNA silencing is enhanced by the graft itself. We can foresee interesting practical implications of the approach described in this paper.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Competing Interests: The authors have declared that no competing interests exist. Conceived and designed the experiments: RS TM RK DG. Performed the experiments: RS TM. Analyzed the data: RS TM RK DG. Contributed reagents/materials/analysis tools: RS TM RK. Wrote the paper: RS TM RK DG.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0141319