Revolutionizing Molecular cloning: Introducing FastCloneAssist, a Streamlined Python tool for optimizing primer design in restriction & ligation-independent PCR cloning

FastCloning, a paradigm shift in PCR cloning, has streamlined the process by eliminating laborious, multi-step traditional methods. This innovative technique, pioneered by Li et al. (2011), utilizes overlapping PCR primers and DpnI digestion for seamless integration of insert DNA into any desired ve...

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Bibliographic Details
Published in:	PloS one Vol. 20; no. 3; p. e0306950
Main Authors:	Singh, Pradip Kumar, Donnenberg, Michael S.
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 13.03.2025 Public Library of Science (PLoS)
Subjects:	Applications software Automation Bioinformatics Biology and Life Sciences Boxes Cloning Cloning, Molecular - methods Cloud computing Computer and Information Sciences Deoxyribonucleic acid Design Design optimization DNA DNA Primers - genetics Engineering and Technology Genetic Vectors - genetics Melt temperature Melting Methods Physical Sciences Polymerase chain reaction Polymerase Chain Reaction - methods Python (Programming language) Research and Analysis Methods Software United States
ISSN:	1932-6203, 1932-6203
Online Access:	Get full text
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