Involvement of microRNA-146a in diabetic peripheral neuropathy through the regulation of inflammation

Recent evidence has shown the involvement of inflammation in the development of diabetic peripheral neuropathy (DPN). MicroRNA-146a (miR-146a) is closely involved in the inflammatory response. However, the role of miR-146a in the inflammatory reaction in DPN has not been clarified. This study was de...

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Vydané v:	Drug design, development and therapy Ročník 12; s. 171 - 177
Hlavní autori:	Feng, Yonghao, Chen, Long, Luo, Qiong, Wu, Men, Chen, Yinghui, Shi, Xiaohong
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	New Zealand Dove Medical Press Limited 01.01.2018 Taylor & Francis Ltd Dove Medical Press
Predmet:	Animals B cells Beta cells Biomarkers Care and treatment Control Correlation analysis Cytokines Cytokines - biosynthesis Cytokines - genetics Development and progression Diabetes Diabetes mellitus Diabetes mellitus (non-insulin dependent) Diabetes Mellitus, Experimental - complications Diabetes Mellitus, Type 2 - complications Diabetes therapy Diabetic neuropathies Diabetic Neuropathies - genetics Diabetic Neuropathies - physiopathology Diabetic neuropathy diabetic peripheral neuropathy Feeds Gene expression Genetic aspects Health aspects Hyperglycemia IL-1β Immune response Immunoassay Inflammation Inflammation - genetics Inflammation - physiopathology Inflammation Mediators - metabolism Inflammatory response Interleukin 1 Interleukins Laboratory animals Male Metabolism MicroRNA microRNA-146a MicroRNAs MicroRNAs - genetics miRNA Necrosis Nerve conduction Nerves Nervous system Nervous tissues Neural Conduction NF-kappa B - genetics NF-κB NF-κB protein Original Research Pathogenesis Peripheral neuropathy Polymerase chain reaction Proteins Rats Rats, Sprague-Dawley Ribonucleic acid RNA Sciatic nerve Sciatic Nerve - physiopathology Sciatic Neuropathy - genetics Sciatic Neuropathy - physiopathology Streptozocin Tumor necrosis factor-TNF Tumor necrosis factor-α Tumors Type 2 diabetes type II diabetes mellitus Velocity United States > US China microRNA-146a type 2 diabetes mellitus NF-κB diabetic peripheral neuropathy cytokines
ISSN:	1177-8881, 1177-8881
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Shrnutí:	Recent evidence has shown the involvement of inflammation in the development of diabetic peripheral neuropathy (DPN). MicroRNA-146a (miR-146a) is closely involved in the inflammatory response. However, the role of miR-146a in the inflammatory reaction in DPN has not been clarified. This study was designed to explore the role of miR-146a in the regulation of inflammatory responses in DPN. Rats were randomly divided into three groups (n=6 per group): control group, type 2 diabetes mellitus (T2DM) group and DPN group. T2DM and DPN rats were intraperitoneally injected with streptozotocin. Sciatic nerve conduction velocity (NCV) was determined at the 6th week and the 12th week in each group. The expression of microRNAs was detected by quantitative real-time polymerase chain reaction in three sciatic nerves for each group of rats. Expression of inflammatory cytokines in nerve tissues and plasma was measured by Western blot and Bio-Plex Pro™ assays. The NCV and expression levels of miR-146a in the DPN group were significantly decreased ( <0.01) compared to the other two groups. Expression of tumor necrosis factor alpha (TNF-α), interleukin 1 beta (IL-1β) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in the DPN group was significantly increased compared with the control and T2DM groups ( <0.01). Pearson's correlation analysis showed that the expression level of miR-146a was negatively correlated with the levels of IL-1β, TNF-α and NF-κB. miR-146a is involved in the pathogenesis of DPN, and its expression level is closely related to the inflammatory responses that aggravate sciatic nerve injuries.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	1177-8881 1177-8881
DOI:	10.2147/DDDT.S157109