BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis

Enhancers, critical determinants of cellular identity, are commonly recognized by correlative chromatin marks and gain-of-function potential, although only loss-of-function studies can demonstrate their requirement in the native genomic context. Previously, we identified an erythroid enhancer of hum...

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Veröffentlicht in:	Nature (London) Jg. 527; H. 7577; S. 192 - 197
Hauptverfasser:	Canver, Matthew C., Smith, Elenoe C., Sher, Falak, Pinello, Luca, Sanjana, Neville E., Shalem, Ophir, Chen, Diane D., Schupp, Patrick G., Vinjamur, Divya S., Garcia, Sara P., Luc, Sidinh, Kurita, Ryo, Nakamura, Yukio, Fujiwara, Yuko, Maeda, Takahiro, Yuan, Guo-Cheng, Zhang, Feng, Orkin, Stuart H., Bauer, Daniel E.
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	London Nature Publishing Group UK 12.11.2015 Nature Publishing Group
Schlagworte:	13/100 13/109 13/31 38/22 38/70 38/77 38/88 45/23 49/47 631/1647/1511 631/208/200 631/337/572/2102 64/60 692/308/2056 Animals Base Sequence Carrier Proteins - genetics Cells, Cultured Clustered Regularly Interspaced Short Palindromic Repeats - genetics CRISPR-Associated Proteins - metabolism CRISPR-Cas Systems - genetics DNA methylation DNA-Binding Proteins Enhancer Elements, Genetic - genetics Erythroblasts - metabolism Fetal Hemoglobin - genetics Gene expression Genetic diversity Genetic Engineering Genome - genetics Genomes Genomics Humanities and Social Sciences Humans Methods Mice Molecular Sequence Data multidisciplinary Mutagenesis Mutagenesis - genetics Nuclear Proteins - genetics Organ Specificity Physiological aspects Repressor Proteins Reproducibility of Results RNA, Guide, CRISPR-Cas Systems - genetics Science Species Specificity Studies
ISSN:	0028-0836, 1476-4687, 1476-4687
Online-Zugang:	Volltext
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