Importance of potassium ions for ribosome structure and function revealed by long-wavelength X-ray diffraction

The ribosome, the largest RNA-containing macromolecular machinery in cells, requires metal ions not only to maintain its three-dimensional fold but also to perform protein synthesis. Despite the vast biochemical data regarding the importance of metal ions for efficient protein synthesis and the incr...

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Vydané v:	Nature communications Ročník 10; číslo 1; s. 2519 - 12
Hlavní autori:	Rozov, Alexey, Khusainov, Iskander, El Omari, Kamel, Duman, Ramona, Mykhaylyk, Vitaliy, Yusupov, Marat, Westhof, Eric, Wagner, Armin, Yusupova, Gulnara
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	London Nature Publishing Group UK 07.06.2019 Nature Publishing Group Nature Portfolio
Predmet:	631/337/574/1789 631/535/1266 639/638/92/321/1156 Biochemistry, Molecular Biology Cations Chemical synthesis Cryoelectron Microscopy Crystallization Crystallography Crystallography, X-Ray Decoding Electron microscopy Escherichia coli Humanities and Social Sciences Ions Life Sciences Macromolecules Metal ions multidisciplinary Nucleic acids Potassium Potassium - metabolism Protein Biosynthesis Protein Conformation Protein folding Protein synthesis Proteins Ribonucleic acid Ribosomes - metabolism Ribosomes - ultrastructure RNA RNA, Transfer - metabolism Science Science (multidisciplinary) Structural Biology Structural integrity Structure-function relationships Thermus thermophilus - metabolism Wavelength X-ray crystallography X-Ray Diffraction
ISSN:	2041-1723, 2041-1723
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Popis
Shrnutí:	The ribosome, the largest RNA-containing macromolecular machinery in cells, requires metal ions not only to maintain its three-dimensional fold but also to perform protein synthesis. Despite the vast biochemical data regarding the importance of metal ions for efficient protein synthesis and the increasing number of ribosome structures solved by X-ray crystallography or cryo-electron microscopy, the assignment of metal ions within the ribosome remains elusive due to methodological limitations. Here we present extensive experimental data on the potassium composition and environment in two structures of functional ribosome complexes obtained by measurement of the potassium anomalous signal at the K-edge, derived from long-wavelength X-ray diffraction data. We elucidate the role of potassium ions in protein synthesis at the three-dimensional level, most notably, in the environment of the ribosome functional decoding and peptidyl transferase centers. Our data expand the fundamental knowledge of the mechanism of ribosome function and structural integrity. Metal ions play essential roles in myriads of biological processes, from catalytic co-factors to supporting protein and nucleic acid structures. Here the authors use long-wavelength X-ray diffraction to locate hundreds of potassium ions taking part in the formation of rRNA tertiary structure, mediating rRNA–protein interactions and supporting ribosomal protein structures and function.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 PMCID: PMC6555806
ISSN:	2041-1723 2041-1723
DOI:	10.1038/s41467-019-10409-4