Proteomics informed by transcriptomics identifies novel secreted proteins in Dermacentor andersoni saliva

•Dermacentor andersoni expressed sequence tag (EST) database established from salivary gland transcripts.•Proteomics informed by transcriptomics identifies 677 proteins in tick saliva.•Few saliva proteins have homologs in public databases.•Potential anti-inflammatory drug and tick vaccine targets id...

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Veröffentlicht in:International journal for parasitology Jg. 44; H. 13; S. 1029 - 1037
Hauptverfasser: Mudenda, Lwiindi, Pierlé, Sebastián Aguilar, Turse, Joshua E., Scoles, Glen A., Purvine, Samuel O., Nicora, Carrie D., Clauss, Therese R.W., Ueti, Massaro W., Brown, Wendy C., Brayton, Kelly A.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: England Elsevier Ltd 01.11.2014
Schlagworte:
acaricides
adaptive immunity
Animals
anti-inflammatory agents
Arthropod Proteins - analysis
Arthropod Proteins - biosynthesis
Arthropod Proteins - chemistry
Arthropod Proteins - genetics
bovine anaplasmosis
Cattle
coagulation
Colorado
Databases, Nucleic Acid
Dermacentor - genetics
Dermacentor - physiology
Dermacentor andersoni
Expressed Sequence Tags
Feeding Behavior - physiology
fever
Gene expression
Gene Expression Profiling
gene expression regulation
genes
hematophagy
hemostasis
immunomodulation
immunomodulators
inflammation
Ixodidae
Mass spectrometry
pathogens
protein secretion
proteins
Proteomics
public health
Rocky Mountain spotted fever
Saliva
Saliva - chemistry
salivary glands
Salivary Glands - parasitology
Salivary Glands - physiology
Salivary proteins
Tick
ticks
Transcriptomics
tularemia
vaccines
Colorado
Salivary proteins
Transcriptomics
Proteomics
Tick
Gene expression
Saliva
Mass spectrometry
ISSN:0020-7519, 1879-0135, 1879-0135
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Zusammenfassung:•Dermacentor andersoni expressed sequence tag (EST) database established from salivary gland transcripts.•Proteomics informed by transcriptomics identifies 677 proteins in tick saliva.•Few saliva proteins have homologs in public databases.•Potential anti-inflammatory drug and tick vaccine targets identified. Dermacentor andersoni, known as the Rocky Mountain wood tick, is found in the western United States and transmits pathogens that cause diseases of veterinary and public health importance including Rocky Mountain spotted fever, tularemia, Colorado tick fever and bovine anaplasmosis. Tick saliva is known to modulate both innate and acquired immune responses, enabling ticks to feed for several days without detection. During feeding ticks subvert host defences such as hemostasis and inflammation, which would otherwise result in coagulation, wound repair and rejection of the tick. Molecular characterization of the proteins and pharmacological molecules secreted in tick saliva offers an opportunity to develop tick vaccines as an alternative to the use of acaricides, as well as new anti-inflammatory drugs. We performed proteomics informed by transcriptomics to identify D. andersoni saliva proteins that are secreted during feeding. The transcript data generated a database of 21,797 consensus sequences, which we used to identify 677 proteins secreted in the saliva of D. andersoni ticks fed for 2 and 5days, following proteomic investigations of whole saliva using mass spectrometry. Salivary gland transcript levels of unfed ticks were compared with 2 and 5day fed ticks to identify genes upregulated early during tick feeding. We cross-referenced the proteomic data with the transcriptomic data to identify 157 proteins of interest for immunomodulation and blood feeding. Proteins of unknown function as well as known immunomodulators were identified.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0020-7519
1879-0135
1879-0135
DOI:10.1016/j.ijpara.2014.07.003