R-ChIP for genome-wide mapping of R-loops by using catalytically inactive RNASEH1

Nascent RNA may form a three-stranded structure with DNA, called an R-loop, which has been linked to fundamental biological processes such as transcription, replication and genome instability. Here, we provide a detailed protocol for a newly developed strategy, named R-ChIP, for robust capture of R-...

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Bibliographic Details
Published in:	Nature protocols Vol. 14; no. 5; pp. 1661 - 1685
Main Authors:	Chen, Jia-Yu, Zhang, Xuan, Fu, Xiang-Dong, Chen, Liang
Format:	Journal Article
Language:	English
Published:	England Nature Publishing Group 01.05.2019
Subjects:	Animals Biological activity Cell Line Cell lines Chromatin Chromatin Immunoprecipitation - methods Chromosome Mapping - methods Deoxyribonucleic acid DNA DNA - chemistry DNA - genetics DNA damage DNA structure DNA-directed RNA polymerase Gene expression Gene mapping Genomes Genomic instability Hybrid structures Hybrids Immunoprecipitation Mapping Monoclonal antibodies Mutation Myelodysplastic syndrome R-loops Ribonuclease H - chemistry Ribonuclease H - genetics Ribonuclease H - metabolism Ribonucleic acid RNA RNA - chemistry RNA - genetics RNA polymerase RNA polymerase II Splicing Splicing factors Stability Transcription
ISSN:	1754-2189, 1750-2799, 1750-2799
Online Access:	Get full text
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