Overview and analysis of the polyprotein cleavage sites in the family Potyviridae

SUMMARY The genomes of plant viruses in the family Potyviridae encode large polyproteins that are cut by virus‐encoded proteases into ten mature proteins. Three different types of protease have been identified, each of which cuts at sites with a distinctive sequence pattern. The experimental evidenc...

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Vydáno v:	Molecular plant pathology Ročník 6; číslo 4; s. 471 - 487
Hlavní autoři:	Adams, M.J, Antoniw, J.F, Beaudoin, F
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Oxford, UK Blackwell Science Ltd 01.07.2005 Blackwell
Témata:	amino acid sequences Biological and medical sciences Fundamental and applied biological sciences. Psychology literature reviews molecular sequence data Phytopathology. Animal pests. Plant and forest protection plant viruses Plant viruses and viroids polypeptides Potyviridae proteinases Systematics. Structure, properties and multiplication. Genetics viral proteins Virus Peptidases Plant pathogen Enzyme Cleavage site Polyprotein Hydrolases Review Potyviridae
ISSN:	1464-6722, 1364-3703, 1364-3703
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Shrnutí:	SUMMARY The genomes of plant viruses in the family Potyviridae encode large polyproteins that are cut by virus‐encoded proteases into ten mature proteins. Three different types of protease have been identified, each of which cuts at sites with a distinctive sequence pattern. The experimental evidence for this specificity is reviewed and the cleavage site patterns are compiled for all sequenced species within the family. Seven of the nine cleavage sites in each species are cut by the viral NIa‐Pro and patterns around these sites are related where possible to the active site–substrate interactions recently deduced following the resolution of the crystal structure of Tobacco etch virus (TEV) NIa‐Pro (Phan et al., 2002. J. Biol. Chem. 277, 50564–50572). In particular, a revised series of cleavage sites for Sweet potato mild mottle virus (genus Ipomovirus) is proposed with a conserved His at the P1 position. This is supported by homology modelling studies using the TEV structure as a template. The data also provide a standard to correct the annotation of some other published sequences and to help predict these sites in further virus sequences as they become available. Comprehensive data for all sequences of each virus in the family, together with some summaries, have been made available at http://www.rothamsted.bbsrc.ac.uk/ppi/links/pplinks/potycleavage/index.html.
Bibliografie:	http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1364-3703/issues ArticleID:MPP296 ark:/67375/WNG-SFQMTBKR-0 istex:F07F3C889D6B6A63D22FE2DE02EC1CD88D7DE118 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 ObjectType-Review-3 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	1464-6722 1364-3703 1364-3703
DOI:	10.1111/j.1364-3703.2005.00296.x